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Journal / Biology / CRISPR-Cas9介导的斑马鱼心脏中的精确敲入编辑…
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CRISPR-Cas9介导的斑马鱼心脏中的精确敲入编辑
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CRISPR-Cas9介导的斑马鱼心脏中的精确敲入编辑

Article DOI: 10.3791/64209-v • 06:52 min • September 13th, 2022
September 13th, 2022 •
1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1
1Department of Biomedical Physiology and Kinesiology, Simon Fraser University

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该协议描述了一种使用CRISPR-Cas9技术促进斑马鱼胚胎精确敲入编辑的方法。提出了一个表型管道，以证明这些技术对长QT综合征相关基因变异进行建模的适用性。

Tags

生物学，第187期，CRISPR-Cas9，斑马鱼，同源定向修复，hERG，长QT综合征
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