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CRISPR-Cas9-medierade exakta knock-in-redigeringar i zebrafiskhjärtan
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CRISPR-Cas9-medierade exakta knock-in-redigeringar i zebrafiskhjärtan

Article DOI: 10.3791/64209-v • 06:52 min • September 13th, 2022
September 13th, 2022 •
1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1
1Department of Biomedical Physiology and Kinesiology, Simon Fraser University

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Detta protokoll beskriver ett tillvägagångssätt för att underlätta exakta knock-in-redigeringar i zebrafiskembryon med CRISPR-Cas9-teknik. En fenotypningspipeline presenteras för att demonstrera tillämpligheten av dessa tekniker för att modellera en lång QT-syndromassocierad genvariant.

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Biologi Utgåva 187 CRISPR-Cas9 zebrafisk homologistyrd reparation hERG Långt QT-syndrom
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