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Journal / Neuroscience / In vivo Imaging del calcio a campo largo e a due…
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Neuroscience

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JoVE Journal Neuroscience
In vivo Imaging del calcio a campo largo e a due fotoni da un topo utilizzando una grande finestra cranica
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In vivo Imaging del calcio a campo largo e a due fotoni da un topo utilizzando una grande finestra cranica

Article DOI: 10.3791/64224-v • 06:45 min • August 4th, 2022
August 4th, 2022 •
1, 2,3, 4, 2,3, 1
1Department of Neurophysiology, Faculty of Medicine, University of Yamanashi, 2Department of Neuropharmacology, Faculty of Medicine, University of Yamanashi, 3Yamanashi GLIA center, Interdisciplinary Graduate School of Medicine, University of Yamanashi, 4Department of Neurochemistry, Graduate School of Medicine, The University of Tokyo

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Il presente protocollo descrive la realizzazione di una grande finestra cranica (6 x 3 mm2) utilizzando un involucro alimentare, silicone trasparente e vetro di copertura. Questa finestra cranica consente esperimenti di imaging di calcio a campo largo e a due fotoni in vivo nello stesso topo.

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Neuroscienze Numero 186
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