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JoVE Journal Neuroscience
In Vivo Wide-Field og Two-Photon Calcium Imaging fra en mus ved hjelp av et stort kranialt vindu
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In Vivo Wide-Field og Two-Photon Calcium Imaging fra en mus ved hjelp av et stort kranialt vindu

Article DOI: 10.3791/64224-v • 06:45 min • August 4th, 2022
August 4th, 2022 •
1, 2,3, 4, 2,3, 1
1Department of Neurophysiology, Faculty of Medicine, University of Yamanashi, 2Department of Neuropharmacology, Faculty of Medicine, University of Yamanashi, 3Yamanashi GLIA center, Interdisciplinary Graduate School of Medicine, University of Yamanashi, 4Department of Neurochemistry, Graduate School of Medicine, The University of Tokyo

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Den nåværende protokollen beskriver å lage et stort (6 x 3 mm2) kranialvindu ved hjelp av matfolie, gjennomsiktig silikon og dekkglass. Dette kraniale vinduet tillater in vivo vidfelt og to-foton kalsiumavbildningseksperimenter i samme mus.

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