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Journal / Neuroscience / In vivo Imagem de cálcio de campo amplo e de dois …
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Neuroscience

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JoVE Journal Neuroscience
In vivo Imagem de cálcio de campo amplo e de dois fótons de um rato usando uma grande janela craniana
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In vivo Imagem de cálcio de campo amplo e de dois fótons de um rato usando uma grande janela craniana

Article DOI: 10.3791/64224-v • 06:45 min • August 4th, 2022
August 4th, 2022 •
1, 2,3, 4, 2,3, 1
1Department of Neurophysiology, Faculty of Medicine, University of Yamanashi, 2Department of Neuropharmacology, Faculty of Medicine, University of Yamanashi, 3Yamanashi GLIA center, Interdisciplinary Graduate School of Medicine, University of Yamanashi, 4Department of Neurochemistry, Graduate School of Medicine, The University of Tokyo

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O presente protocolo descreve a confecção de uma grande janela craniana (6 x 3 mm2) usando envoltório de alimentos, silicone transparente e vidro de cobertura. Esta janela craniana permite experiências de imagem de cálcio in vivo de campo largo e de dois fótons no mesmo rato.

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Neurociência Edição 186
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