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JoVE Journal Biology
En platform med høj kapacitet til kultur og 3D-billeddannelse af organoider
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En platform med høj kapacitet til kultur og 3D-billeddannelse af organoider

Article DOI: 10.3791/64405-v • 07:42 min • October 14th, 2022
October 14th, 2022 •
1,2, 1, 1, 1, 1, 1, 1,3,4, 1,5
1Mechanobiology Institute (MBI), National University of Singapore, 2Biomedical Engineering Department, National University of Singapore, 3Department of Biological Sciences, National University of Singapore, 4IRL 3639 CNRS, 5Department of Microbiology & Immunology, Immunology Translational Research Program, Yong Loo Lin School of Medicine, National University of Singapore

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Dette papir præsenterer en fabrikationsprotokol for en ny type kultursubstrat med hundredvis af mikrobeholdere pr. mm2, hvor organoider kan dyrkes og observeres ved hjælp af mikroskopi med høj opløsning. Protokollerne for cellesåning og immunfarvning er også detaljerede.

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Biologi nr. 188
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