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Journal / Immunology and Infection / Detecção de Células T Polifuncionais em Crianças…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Detecção de Células T Polifuncionais em Crianças Vacinadas com Encefalite Japonesa através da Técnica de Citometria de Fluxo
Assembly and Purification of Prototype Foamy Virus Intasomes
Journal (Immunology and Infection)
Assembly and Purification of Prototype Foamy Virus Intasomes
Cell Membrane Repair Assay Using a Two-photon Laser Microscope
Journal (Immunology and Infection)
Cell Membrane Repair Assay Using a Two-photon Laser Microscope
 
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Detecção de Células T Polifuncionais em Crianças Vacinadas com Encefalite Japonesa através da Técnica de Citometria de Fluxo

Article DOI: 10.3791/64671-v • 09:37 min • September 23rd, 2022
September 23rd, 2022 •
*1,2, *1,2,3, 1,2, 1,2, 1,2, 4, 1,2, 1,2
1Beijing Key Laboratory of Pediatric Respiratory Infectious Diseases, Key Laboratory of Major Diseases in Children, Ministry of Education, National Clinical Research Center for Respiratory Diseases, Laboratory of Infection and Virology, Beijing Pediatric Research Institute, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s Health, 2Research Unit of Critical Infection in Children, 2019RU016, Chinese Academy of Medical Sciences, 3Department of Pediatric Rehabilitation, Beijing Boai Hospital, School of Rehabilitation Medicine, Capital Medical University, China Rehabilitation Research Center, 4Center of Excellence (Beijing), Becton Dickinson Medical Devices (Shanghai) Co Ltd
* These authors contributed equally

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O presente protocolo combina estimulação ex vivo e citometria de fluxo para analisar perfis polifuncionais de células T (TPF) em células mononucleares do sangue periférico (PBMCs) em crianças vacinadas com o vírus da encefalite japonesa (JEV). O método de detecção e o esquema de cores da citometria de fluxo de TPFs específicos do JEV foram testados para fornecer uma referência para estudos semelhantes.

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Imunologia e Infecção Edição 187 Células T polifuncionais encefalite japonesa vacinação citometria de fluxo
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