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JoVE Journal Biology
Langtidsdyrkning af individuelle Caenorhabditis elegans på faste medier til langsgående fluorescensovervågning og aversive indgreb
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Langtidsdyrkning af individuelle Caenorhabditis elegans på faste medier til langsgående fluorescensovervågning og aversive indgreb

Article DOI: 10.3791/64682-v • 07:25 min • December 2nd, 2022
December 2nd, 2022 •
*1, *1, 1, 1, 1, 1, 1, 1, 1
1Department of Molecular & Cellular Biology, University of Arizona, Tucson
* These authors contributed equally

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Her præsenterer vi en protokol til dyrkning af isolerede individuelle nematoder på faste medier til livslang fysiologisk parametersporing og fluorescenskvantificering. Dette kultursystem omfatter en palmitinsyrebarriere omkring enkeltormbrønde for at forhindre dyr i at flygte, hvilket tillader anvendelse af aversive indgreb, herunder patogene bakterier og kemiske stressorer.

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Tilbagetrækning nummer 190
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