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Langsiktig kultur av individuelle Caenorhabditis elegans på faste medier for langsgående fluorescensovervåking og aversive intervensjoner
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Langsiktig kultur av individuelle Caenorhabditis elegans på faste medier for langsgående fluorescensovervåking og aversive intervensjoner

Article DOI: 10.3791/64682-v • 07:25 min • December 2nd, 2022
December 2nd, 2022 •
*1, *1, 1, 1, 1, 1, 1, 1, 1
1Department of Molecular & Cellular Biology, University of Arizona, Tucson
* These authors contributed equally

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Her presenterer vi en protokoll til dyrkningsisolerte individuelle nematoder på faste medier for livslang fysiologisk parametersporing og fluorescenskvantifisering. Dette kultursystemet inkluderer en palmitinsyrebarriere rundt enkeltormbrønner for å forhindre at dyr flykter, noe som tillater bruk av aversive inngrep, inkludert patogene bakterier og kjemiske stressorer.

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