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Agrobacterium tumefaciens-mediert genetisk transformasjon av smalbladet plantain
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Agrobacterium tumefaciens-mediert genetisk transformasjon av smalbladet plantain

Article DOI: 10.3791/64777-v • 07:50 min • March 17th, 2023
March 17th, 2023 •
1, 1, 1, 2, 1, 3, 4, 1
1Department of Agronomy, Center for Plant Biology, Purdue University, 2Department of Biology, Purdue University, 3Department of Horticulture, Faculty of Agricultural Sciences, University of the Punjab, 4Department of Botany and Plant Pathology, Center for Plant Biology, Purdue University

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På grunn av sin allsidige anvendelse som modellart i ulike fagområder, er det behov for en genetisk transformasjonsverktøykasse i smalbladet plantain (Plantago lanceolata). Her, ved bruk av Agrobacterium tumefaciens-mediert transformasjon, presenteres en protokoll som resulterer i stabile transgene linjer med en transformasjonseffektivitet på 20%.

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