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JoVE Journal Environment
废水和空气样本中SARS-CoV-2 RNA的定量和全基因组表征
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废水和空气样本中SARS-CoV-2 RNA的定量和全基因组表征

Article DOI: 10.3791/65053-v • 09:26 min • June 30th, 2023
June 30th, 2023 •
1,2,3, 4,5,6, 3, 3, 1,2, 1,2, 1,2, 2,3, 4,5, 1,2
1Institute of Sustainable Processes, University of Valladolid, 2Department of Chemical Engineering and Environmental Technology, University of Valladolid, 3Department of Public Health Microbiology, National Laboratory of Health, Environment and Food, 4ICBAS - School of Medicine and Biomedical Sciences, Porto University, 5Epidemiology Research Unit (EPIUnit), Instituto de Saúde Pública da Universidade do Porto, 6Laboratório para a Investigação Integrativa e Translacional em Saúde Populacional (ITR)

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该协议旨在量化废水和空气样本中的SARS-CoV-2 RNA，用于基于废水的流行病学研究，并评估室内和室外气溶胶中SARS-CoV-2的暴露风险。该协议还描述了用于 SARS-CoV-2 全基因组表征的平铺扩增子长模板测序方法。

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环境科学，第 196 期，SARS-CoV-2，RNA，RT-qPCR，测序，关注的变异株
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