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JoVE Journal Environment
廃水および大気サンプル中のSARS-CoV-2 RNAの定量と全ゲノム特性評価
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廃水および大気サンプル中のSARS-CoV-2 RNAの定量と全ゲノム特性評価

Article DOI: 10.3791/65053-v • 09:26 min • June 30th, 2023
June 30th, 2023 •
1,2,3, 4,5,6, 3, 3, 1,2, 1,2, 1,2, 2,3, 4,5, 1,2
1Institute of Sustainable Processes, University of Valladolid, 2Department of Chemical Engineering and Environmental Technology, University of Valladolid, 3Department of Public Health Microbiology, National Laboratory of Health, Environment and Food, 4ICBAS - School of Medicine and Biomedical Sciences, Porto University, 5Epidemiology Research Unit (EPIUnit), Instituto de Saúde Pública da Universidade do Porto, 6Laboratório para a Investigação Integrativa e Translacional em Saúde Populacional (ITR)

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このプロトコルは、廃水および空気サンプル中の SARS-CoV-2 RNA を定量化して、廃水ベースの疫学研究に使用し、屋内および屋外のエアロゾル中の SARS-CoV-2 への曝露リスクを評価することを目的としています。このプロトコルでは、SARS-CoV-2の全ゲノム特性評価のためのタイルアンプリコンロングテンプレートシーケンシングアプローチについても説明します。

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Environmental Sciences SARS-CoV-2 RNA RT-qPCR シーケンシング 懸念される変異株
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