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JoVE Journal Environment
Kvantificering og helgenomkarakterisering af SARS-CoV-2 RNA i spildevands- og luftprøver
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Kvantificering og helgenomkarakterisering af SARS-CoV-2 RNA i spildevands- og luftprøver

Article DOI: 10.3791/65053-v • 09:26 min • June 30th, 2023
June 30th, 2023 •
1,2,3, 4,5,6, 3, 3, 1,2, 1,2, 1,2, 2,3, 4,5, 1,2
1Institute of Sustainable Processes, University of Valladolid, 2Department of Chemical Engineering and Environmental Technology, University of Valladolid, 3Department of Public Health Microbiology, National Laboratory of Health, Environment and Food, 4ICBAS - School of Medicine and Biomedical Sciences, Porto University, 5Epidemiology Research Unit (EPIUnit), Instituto de Saúde Pública da Universidade do Porto, 6Laboratório para a Investigação Integrativa e Translacional em Saúde Populacional (ITR)

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Denne protokol har til formål at kvantificere SARS-CoV-2 RNA i spildevands- og luftprøver, der skal anvendes til spildevandsbaserede epidemiologiske undersøgelser, og at vurdere eksponeringsrisikoen for SARS-CoV-2 i indendørs og udendørs aerosoler. Denne protokol beskriver også en flisebelagt amplicon langskabelonsekventeringstilgang til SARS-CoV-2 helgenomkarakterisering.

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Miljøvidenskab udgave 196 SARS-CoV-2 RNA RT-qPCR sekventering varianter af bekymring
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