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Journal / Medicine / Humane Endothelzellisolierung der Vena saphena…
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JoVE Journal Medicine
Humane Endothelzellisolierung der Vena saphena und Exposition bei kontrollierter Scherspannung und Dehnung
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Humane Endothelzellisolierung der Vena saphena und Exposition bei kontrollierter Scherspannung und Dehnung

Article DOI: 10.3791/65122-v • 09:10 min • April 21st, 2023
April 21st, 2023 •
*1,2, *1, 1, 1, 1, 1, 1
1Instituto do Coraçao (InCor), Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, 2Cardiology Research Group, Faculty VI Medicine and Health Sciences, Carl von Ossietzky University of Oldenburg
* These authors contributed equally

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Wir beschreiben ein Protokoll zur Isolierung und Kultivierung humaner Endothelzellen der Vena saphena (hSVECs). Wir stellen auch detaillierte Methoden zur Erzeugung von Scherspannung und Dehnung zur Verfügung, um mechanische Spannungen in hSVECs zu untersuchen.

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