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Aislamiento de células endoteliales de venas safenas humanas y exposición a niveles controlados de esfuerzo cortante y estiramiento
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Aislamiento de células endoteliales de venas safenas humanas y exposición a niveles controlados de esfuerzo cortante y estiramiento

Article DOI: 10.3791/65122-v • 09:10 min • April 21st, 2023
April 21st, 2023 •
*1,2, *1, 1, 1, 1, 1, 1
1Instituto do Coraçao (InCor), Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, 2Cardiology Research Group, Faculty VI Medicine and Health Sciences, Carl von Ossietzky University of Oldenburg
* These authors contributed equally

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Describimos un protocolo para aislar y cultivar células endoteliales de venas safenas humanas (hSVECs). También proporcionamos métodos detallados para producir esfuerzo cortante y estiramiento para estudiar el estrés mecánico en hSVEC.

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Este mes en JoVE Número 194
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