Silent Lacunes

Silent lacunes are small, chronic brain lesions caused by infarction in deep tissue, often without recognizable stroke symptoms, yet they may influence behavior and cognition. They typically develop when disease of small penetrating arteries, including arteriolosclerosis associated with hypertension, reduces blood flow and produces a tiny subcortical infarct that later appears as a cavity on magnetic resonance imaging. In behavioral research, identifying silent lacunes helps relate cerebrovascular injury to changes in attention, executive function, mood, and motor control. Their presence can also indicate broader small-vessel disease and increased risk of future symptomatic stroke, supporting early assessment of vascular risk factors.

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Research

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Gliding Motility in Phormidium lacuna Filaments

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2025

Source: Weber, N., et al., Natural Transformation, Protein Expression, and Cryoconservation of the Filamentous Cyanobacterium Phormidium lacuna. J. Vis. Exp. (2022)This video demonstrates the procedure to assess gliding motility in Phormidium lacuna by homogenizing filament cultures to create a uniform suspension, preparing a Petri plate assay setup over a microscope slide, and visualizing filament movement.

Presynaptically Silent Synapses Studied with Light Microscopy

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Cited by 14 •

2010

Glutamatergic synapses can switch from an active mode to a silent mode. We demonstrate that presynaptic activity status in dissociated culture of rodent neurons is visualized using a fixable form of the FM1-43 dye to visualize active synapses and immunostaining with vGluT-1 antibody to visualize all glutamate synapses.

Natural Transformation and GFP Expression in the Filamentous Cyanobacterium Phormidium lacuna

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2025

Source: Weber, N., et al., Natural Transformation, Protein Expression, and Cryoconservation of the Filamentous Cyanobacterium Phormidium lacuna. J. Vis. Exp. (2022)This video describes a procedure for performing natural transformation in filamentous cyanobacteria using plasmid DNA to achieve stable GFP expression and antibiotic resistance through homologous recombination.

Measuring the Contralateral Silent Period Induced by Transcranial Magnetic Stimulation

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2025

This video demonstrates a technique for measuring the contralateral silent period (cSP) using transcranial magnetic stimulation. Position the patient's hand prone and place an electrode on the first dorsal interosseous (FDI) muscle. Then, place a force-measuring device between the thumb and index finger. Identify the primary motor cortex (M1) region of the brain. Maintain a standardized pressure on the force-measuring device and stimulate the M1 region to record the muscle activity, termed the...

Evaluation of the Cognitive Performance of Hypertensive Patients with Silent Cerebrovascular Lesions

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Cited by 3 •

2021

Here, we present a protocol to assess whether various types of silent cerebrovascular lesions are differentially associated with deficits in certain cognitive domains in a cohort of 398 hypertensive elderly Chinese, using a combination of neuropsychological tests and multi-sequence 3T MRI scanning.

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