Temporal Bone Window
Temporal Bone Window - Related Videos
08:41
Femur Window Chamber Model for In Vivo Cell Tracking in the Murine Bone Marrow
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Cited by 8 •
2016
The protocol describes a novel murine femur window chamber model that can be used to track movement of cells in the femoral bone marrow in vivo. Intravital multiphoton fluorescence microscopy is used to image three components of the femoral bone marrow (vasculature, collagen matrix, and neutrophils) over time.
11:52
Temporal Ordering of Dynamic Expression Data from Detailed Spatial Expression Maps
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2017
The segmentation clock drives oscillatory gene expression across the pre-somitic mesoderm (PSM). Dynamic Notch activity is key to this process. We use imaging and computational analyses to extract temporal dynamics from spatial expression data to demonstrate that Delta ligand and Notch receptor expression oscillate in the vertebrate PSM.
06:42
Extracting the Cochlea from a Human Temporal Bone: A Cadaveric Protocol
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2023
This article presents a reliable method for extracting the human cochlea from the cadaver temporal bone by drill-out while following distinct anatomical landmarks.
03:15
Calvarial Window Generation in Mouse Model: A Surgical Procedure to Create Window in the Top Part of the Mouse Skull
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2023
In this video, we describe the surgical procedure to create a calvarial window for accessing the brain of a mouse model.
09:49
A Polished and Reinforced Thinned-skull Window for Long-term Imaging of the Mouse Brain
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Cited by 128 •
2012
We present a method to form an imaging window in the mouse skull that spans millimeters and is stable for months without inflammation of the brain. This method is well suited for longitudinal studies of blood flow, cellular dynamics, and cell/vascular structure using two-photon microscopy.
FAQs
The preparation exposes the cochlea and nearby tissues while maintaining experimental control over the observation site. This access allows researchers to select optical imaging, electrophysiology, or related measurements according to the biological signal of interest. Because measurements occur in living animals, investigators can examine auditory activity in relation to stimulation, injury, treatment, or behavioral performance.
Stabilizing the exposed region helps maintain a consistent measurement site while researchers observe or record activity from auditory structures. This is particularly important when optical imaging or electrophysiology is used, because both approaches require controlled access to the tissue. A stable preparation also supports comparisons across experimental conditions and, when feasible, observations made at different time points.
Optical imaging and electrophysiology provide complementary ways to monitor activity in the cochlea and nearby tissues. The preparation gives researchers access for observing physiological responses or recording electrical signals, while the chosen method determines how activity is measured. These data can then be related to sound stimulation, auditory function, or changes associated with injury and treatment.
Behavioral testing supplies an outcome that can be compared with cellular or physiological measurements obtained from auditory structures. For example, researchers can examine whether tissue responses correspond with hearing-related performance or sound localization. This relationship helps place activity in the cochlea and nearby regions within a broader functional context rather than interpreting physiological signals in isolation.
Establishing the preparation requires carefully thinning or removing the relevant bone, creating access to auditory structures, and stabilizing the exposed region for measurement. Researchers then apply optical imaging, electrophysiology, or another supported technique while maintaining experimental control. Behavioral testing can be incorporated to compare tissue activity with hearing or sound-localization outcomes.
Longitudinal studies are useful when the research question concerns how auditory responses change over time. Repeated observations can track responses to sound stimulation, injury, treatment, or other experimental changes, while behavioral measurements provide functional context. This design can reveal patterns that a single time point would not capture, including evolving relationships between auditory physiology and behavior.