Metabolic Alterations Study
Metabolic Alterations Study - Related Videos
05:08
Plate-based Large-scale Cultivation of Caenorhabditis elegans: Sample Preparation for the Study of Metabolic Alterations in Diabetes
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Cited by 5 •
2018
This protocol describes a method for the large-scale cultivation of Caenorhabditis elegans on solid media. As an alternative to liquid culture, this protocol allows obtaining parameters of different scales under plate-based cultivation. This increases the comparability of results by omitting the morphological and metabolic differences between liquid and solid media culture.
06:04
LC-MS Analysis of Human Platelets as a Platform for Studying Mitochondrial Metabolism
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Cited by 7 •
2016
Here we show isolated human platelets can be used as an accessible ex vivo model to study metabolic adaptations in response to the complex I inhibitor rotenone. This approach employs isotopic tracing and relative quantification by liquid chromatography-mass spectrometry and can be applied to a variety of study designs.
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Metabolic Profile Analysis of Zebrafish Embryos
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Cited by 42 •
2013
Zebrafish represent a powerful vertebrate model that has been under-utilised for metabolic studies. Here we describe a rapid way to measure the in vivo metabolic profile of developing zebrafish that allows the comparison of different mitochondrial function parameters between genetically or pharmacologically manipulated embryos, thereby increasing the applicability of this organism.
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What is Metabolism?
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2019
Overview Metabolism represents all of the chemical activity in a cell, including reactions that build molecules (anabolism) and those that break molecules down (catabolism). Anabolic reactions require energy, whereas catabolic reactions provide it. Thus, metabolism describes how cells transform energy through a variety of chemical reactions, which are often made more efficient with the help of enzymes. Metabolism Is the Sum of All of the Chemical Reactions That Take Place in an Organism
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An Introduction to Cell Metabolism
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2023
In cells, critical molecules are either built by joining together individual units like amino acids or nucleotides, or broken down into smaller components. Respectively, the reactions responsible for this are referred to as anabolic and catabolic. These reactions require or produce energy typically in the form of a “high-energy” molecule called ATP. Together, these processes make up “Cell Metabolism,” and are hallmarks of healthy, living cells.JoVE’s introduction to cell metabolism briefly...
FAQs
Pathway flux shows how rapidly metabolites move through a biochemical pathway, rather than merely indicating their abundance at one moment. In a metabolic alterations study, comparing flux before and after a perturbation can distinguish a change in pathway throughput from a change caused mainly by metabolite accumulation. This helps connect altered nutrient utilization, biosynthesis, or energy production to the underlying cellular response.
Each perturbation can alter metabolism by changing the conditions under which cells, tissues, or organisms use nutrients, synthesize compounds, or generate energy. Comparing profiles before and after the selected genetic, environmental, or physiological change reveals which metabolic features respond to that condition. The resulting differences help relate a specific perturbation to biochemical pathway disruption or adaptation.
These measurements provide complementary views of biochemical regulation. Metabolite concentrations show changes in the chemical profile, enzyme activity indicates altered catalytic function, and pathway flux reflects changes in overall pathway throughput. Examining them together can strengthen interpretation of a metabolic shift, helping researchers determine whether altered metabolite levels accompany changes in enzymatic behavior or energy and biosynthetic processing.
A typical workflow compares samples collected before and after a defined genetic, environmental, or physiological perturbation. Researchers may examine cells, tissues, or organisms and measure metabolite concentrations, enzyme activity, or pathway flux. They then compare the resulting metabolic profiles to identify changes in nutrient utilization, biosynthesis, or energy production and relate those changes to the tested condition.
The approach can be applied to cells, tissues, or whole organisms exposed to a specified condition. Relevant comparisons may involve a genetic change, an environmental influence, or a physiological state, with measurements taken before and after the perturbation. Selecting the system and comparison according to the research question allows investigators to assess how metabolism responds across different levels of biological organization.
In biochemistry, these studies can identify biomarkers, clarify disease mechanisms, and evaluate drug effects by linking metabolic profiles with altered cellular function. They can also support strategies intended to restore disrupted metabolic processes. Comparing pathway behavior and metabolite patterns under defined conditions provides evidence for how disease-related dysfunction or treatment exposure affects nutrient use, biosynthesis, and energy production.