Plate-based Large-scale Cultivation of <em>Caenorhabditis elegans</em>: Sample Preparation for the Study of Metabolic Alterations in Diabetes

Katharina Kohl; Thomas Fleming; K&#xFC;bra Acunman; Hans-Peter Hammes; Michael Morcos; Andrea Schlotterer

doi:10.3791/58117

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Plate-based Large-scale Cultivation of Caenorhabditis elegans: Sample Preparation for the Study of Metabolic Alterations in Diabetes

Plate-based Large-scale Cultivation of <em>Caenorhabditis elegans</em>: Sample Preparation for the Study of Metabolic Alterations in Diabetes

JoVE Journal
Biochemistry

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JoVE Journal Biochemistry

Plate-based Large-scale Cultivation of Caenorhabditis elegans: Sample Preparation for the Study of Metabolic Alterations in Diabetes

Plate-based Large-scale Cultivation of Caenorhabditis elegans: Sample Preparation for the Study of Metabolic Alterations in Diabetes

6,325 Views

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05:08 min

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August 24, 2018

DOI:

10.3791/58117-v

DOI:

10.3791/58117-v

•

05:08 min

August 24, 2018

•

6287 Views

Katharina Kohl¹, Thomas Fleming^2,3, Kübra Acunman¹, Hans-Peter Hammes^1,4, Michael Morcos*¹, Andrea Schlotterer*¹

¹5th Medical Department, Medical Faculty Mannheim,Heidelberg University ²Department of Internal Medicine,Heidelberg University ³German Center for Diabetes Research (DZD)⁴European Center for Angioscience, Medical Faculty Mannheim,Heidelberg University

Summary

This protocol describes a method for the large-scale cultivation of Caenorhabditis elegans on solid media. As an alternative to liquid culture, this protocol allows obtaining parameters of different scales under plate-based cultivation. This increases the comparability of results by omitting the morphological and metabolic differences between liquid and solid media culture.