Isogenic Microcolonies
Isogenic Microcolonies - Related Videos
12:52
High-Throughput Live Imaging of Microcolonies to Measure Heterogeneity in Growth and Gene Expression
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Cited by 7 •
2021
Yeast growth phenotypes are precisely measured through highly parallel time-lapse imaging of immobilized cells growing into microcolonies. Simultaneously, stress tolerance, protein expression, and protein localization can be monitored, generating integrated datasets to study how environmental and genetic differences, as well as gene-expression heterogeneity among isogenic cells, modulate growth.
10:23
Isogenic Kidney Glomerulus Chip Engineered from Human Induced Pluripotent Stem Cells
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Cited by 11 •
2022
Presented here is a protocol to engineer a personalized organ-on-a-chip system that recapitulates the structure and function of the kidney glomerular filtration barrier by integrating genetically matched epithelial and vascular endothelial cells differentiated from human induced pluripotent stem cells. This bioengineered system can advance kidney precision medicine and related applications.
12:04
Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis: Fabrication, System Setup, and Operation
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Cited by 75 •
2013
In this protocol the fabrication, setup and basic operation of a microfluidic picoliter bioreactor (PLBR) for single-cell analysis of prokaryotic microorganisms is introduced. Industrially relevant microorganisms were analyzed as proof of principle allowing insights into growth rate, morphology, and phenotypic heterogeneity over certain time periods, hardly possible with conventional methods.
02:28
Extraction of Apoplastic Bacteria Using a Syringe-Based Pressure Method
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2025
Source: Rufián, J. S., et al, Single-Cell Analysis of the Expression of Pseudomonas syringae Genes within the Plant Tissue. J. Vis. Exp. (2022)This video demonstrates a syringe-based pressure method for extracting apoplastic bacteria, which proliferate and form microcolonies within the apoplast, the intercellular space between plant cells. The method yields debris-free bacterial samples suitable for downstream single-cell analysis.
03:50
Candida albicans Biofilm Formation in an In Vivo Mouse Model
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2025
This video demonstrates the development of an in vivo mouse model to study the biofilm formation of Candida albicans in subcutaneous tissue. The experiment involves implanting Candida-infected devices in the animal's back region for biofilm development.
FAQs
They begin with genetically identical starting cells, so differences in growth, differentiation, signaling, or organization can be linked more directly to controlled surroundings rather than inherited variation. Bioengineers can then vary geometry, cell number, or extracellular cues systematically. This experimental separation improves interpretation when testing how defined conditions shape cellular behavior.
Cell number and geometry establish the physical context in which cells grow and interact. Controlling these variables helps researchers examine how spatial arrangement and colony size influence differentiation, signaling, growth, and tissue organization. Consistent microscale layouts also improve reproducibility, allowing measurements from different colonies to be compared under defined experimental conditions.
Extracellular cues provide environmental signals that can modify cellular behavior even when the cells share the same genotype. By controlling these cues, researchers can investigate changes in differentiation, growth, signaling, and organization without introducing genetic differences between groups. This makes the system useful for identifying how surroundings shape coordinated cellular responses.
Their controlled composition and spatial arrangement make it easier to examine interactions among neighboring cells. Because genetic variation is minimized, observed changes in signaling, growth, or organization can be interpreted in relation to colony structure and defined environmental conditions. This supports quantitative analysis of how local cellular interactions contribute to larger patterns of tissue organization.
A typical workflow starts by isolating an individual cell or clonal progenitor, placing it in a microscale compartment or patterned culture environment, and allowing it to proliferate. Researchers control variables such as starting cell number, geometry, and extracellular cues during culture. The resulting colonies can then be evaluated for growth, differentiation, signaling, or organization.
They are useful when researchers need a reproducible cellular system in which environmental conditions can be defined while genetic differences remain limited. Such cultures support comparisons of disease-related behavior or responses to treatments across controlled colony settings. Their small, organized format also enables quantitative examination of how growth, differentiation, signaling, or organization changes under the tested conditions.
Isogenic microcolonies provide a controlled way to study the cellular behaviors that support tissue organization. By adjusting spatial geometry, cell number, and extracellular cues, bioengineers can examine how defined environments affect growth, differentiation, and signaling. The resulting measurements can inform strategies for designing engineered tissues with more predictable organization and behavior.