Plga Nanoparticles
Plga Nanoparticles - Related Videos
12:48
PLGA Nanoparticles Formed by Single- or Double-emulsion with Vitamin E-TPGS
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Cited by 211 •
2013
We describe the production and characterization of nanoparticles and microparticles composed of poly(lactic-co-glycolic acid) using vitamin E-TPGS as an emulsifier. By varying formulation parameters such as the concentration of emulsifier, it is possible to produce nanoparticles with mean diameters ranging from 220 nm to 1.98 µm.
06:47
Formulation of Diblock Polymeric Nanoparticles through Nanoprecipitation Technique
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Cited by 16 •
2011
This article describes a nanoprecipitation method to synthesize polymer-based nanoparticles using diblock co-polymers. We will discuss the synthesis of diblock co-polymers, the nanoprecipitation technique, and potential applications.
08:26
Cell Labeling and Targeting with Superparamagnetic Iron Oxide Nanoparticles
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Cited by 20 •
2015
Targeted cell delivery is useful in a variety of biomedical applications. The goal of this protocol is to use superparamagnetic iron oxide nanoparticles (SPION) to label cells and thereby enable magnetic cell targeting approaches for a high degree of control over cell delivery and localization.
10:27
Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering
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Cited by 4 •
2016
This protocol describes the use of an inertial microfluidics-based buffer exchange strategy to purify micro/nanoparticle engineered cells with efficient depletion of unbound particles.
09:23
Harmonic Nanoparticles for Regenerative Research
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Cited by 1 •
2014
Protocol details are provided for in vitro labeling human embryonic stem cells with second harmonic generating nanoparticles. Methodologies for hESC investigation by multi-photon microscopy and their differentiation into cardiac clusters are also presented.
FAQs
Particle size and polymer composition are major determinants of how PLGA nanoparticles handle cargo. Size can influence cellular uptake, while composition affects how rapidly the polymer matrix undergoes hydrolysis and therefore how cargo is released. Surface properties add another layer by shaping interactions with cells. Adjusting these features helps bioengineers align loading and release behavior with a specific application.
The ester-containing matrix gradually hydrolyzes into lactic acid and glycolic acid. This degradation process provides the basis for gradual cargo release from the carrier. Because degradation behavior depends on formulation characteristics, researchers must evaluate it for each intended use. The resulting kinetics determine whether the particles are suitable for controlled delivery in a given bioengineering system.
Surface properties influence how PLGA nanoparticles interact with cells and can consequently affect cellular uptake. That uptake helps determine where associated cargo can act after delivery. Surface characteristics also contribute to release behavior alongside size and polymer composition. In engineered tissues or disease models, considering these variables together supports better localization and more predictable particle performance.
Encapsulation or association with the particles can protect unstable therapeutic and diagnostic compounds from the surrounding environment. This protective role may help preserve cargo while the carrier delivers it, although the formulation still requires optimization for the compound and application. In bioengineering, that capability is useful when researchers need to combine molecular protection with controlled release or improved localization.
PLGA nanoparticles are used across several bioengineering research areas, including drug delivery, vaccines, gene therapy, and regenerative medicine. The same carrier platform can therefore be adapted to different cargo and biological goals, but its design cannot be assumed to work identically in each setting. Researchers must match particle characteristics and degradation behavior to the requirements of the chosen application.
In engineered tissues and disease models, particle size, surface properties, and polymer composition help determine localization, uptake, and release kinetics. These measurements connect formulation design with biological performance: a particle may carry cargo effectively yet require adjustment if it does not localize appropriately or release its contents at a useful rate. This makes PLGA formulation an application-specific bioengineering problem.
Evaluation commonly focuses on cargo loading, cellular uptake, release kinetics, and localization. Together, these outcomes show whether a formulation can carry the intended compound, interact with relevant cells, release cargo over time, and reach a desired engineered tissue or disease-model site. Examining all four provides a more complete assessment than measuring particle size alone.