Tissue Engineered Constructs
Tissue Engineered Constructs - Related Videos
13:43
Fabrication of Myogenic Engineered Tissue Constructs
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Cited by 11 •
2009
Here, we demonstrate fabrication of collagen-based, tissue constructs containing skeletal myoblasts. These 3-D engineered constructs may be used to replace or repair tissues in vivo. For our purposes, we have designed these as an atrioventricular electrical conduit for the repair of complete heart block[1].
12:18
Magnetic Resonance Elastography Methodology for the Evaluation of Tissue Engineered Construct Growth
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Cited by 16 •
2012
The procedure demonstrates the methodology of magnetic resonance elastography for monitoring the engineered outcome of adipose and osteogenic tissue engineered constructs through noninvasive local assessment of the mechanical properties using microscopic magnetic resonance elastography (μMRE).
09:24
Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets
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Cited by 2 •
2014
For creation of highly organized structures of complex tissue, one must assemble multiple material and cell types into an integrated composite. This combinatorial design incorporates organ-specific layered cell sheets with two distinct biologically-derived materials containing a strong fibrous matrix base, and endothelial cells for enhancing new vessels formation.
12:13
Engineering Fibrin-based Tissue Constructs from Myofibroblasts and Application of Constraints and Strain to Induce Cell and Collagen Reorganization
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Cited by 5 •
2013
This model system starts from a myofibroblast-populated fibrin gel that can be used to study endogenous collagen (re)organization real-time in a nondestructive manner. The model system is very tunable, as it can be used with different cell sources, medium additives, and can be adapted easily to specific needs.
08:03
Treatment of Ligament Constructs with Exercise-conditioned Serum: A Translational Tissue Engineering Model
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Cited by 7 •
2017
We present a model of ligament tissue in which three-dimensional constructs are treated with the human exercise-conditioned serum and analyzed for collagen content, function, and cellular biochemistry.
FAQs
Scaffold architecture shapes how cells adhere, organize, and interact with their surroundings. Its three-dimensional arrangement also affects the movement of oxygen and nutrients through the construct, which can influence cell proliferation, differentiation, and extracellular matrix deposition. Designing architecture alongside mechanical properties helps researchers create constructs that mature appropriately and perform more effectively after implantation or during experimental tissue modeling.
Cells within a three-dimensional construct depend on adequate oxygen and nutrient transport to remain active and develop tissue-specific organization. Limited transport can interfere with proliferation, differentiation, and extracellular matrix production, while effective transport supports maturation throughout the assembly. Researchers therefore consider transport together with scaffold structure when evaluating whether a construct can function beyond its immediate outer regions.
Cell adhesion provides the attachment needed for cells to remain positioned within the biomaterial and organize into a functional arrangement. Controlled biochemical or physical cues then influence how those cells proliferate, differentiate, and deposit extracellular matrix. Together, these factors guide maturation, making them important design variables when researchers seek to reproduce tissue development or generate an implant with organized biological function.
Material selection determines the environment in which cells attach, organize, and produce extracellular matrix, while mechanical properties influence how the construct behaves during development and use. Degradation is also important because changes in the biomaterial can affect cell organization and the evolving tissue. Balancing these characteristics supports construct maturation and improves the potential for integration with host tissue.
Development begins by combining living cells with a selected biomaterial and establishing controlled biochemical or physical cues. Researchers then evaluate cell adhesion, organization, proliferation, differentiation, and extracellular matrix deposition as the construct matures. Scaffold architecture, transport, mechanical behavior, and degradation are assessed throughout this process because each can alter biological performance and the prospects for host-tissue integration.
Researchers use these constructs for several distinct purposes: studying tissue development, modeling disease, testing therapeutics, and developing implants for regenerative medicine. The same design principles apply differently across these settings. A disease model may emphasize controlled biological behavior, whereas an implant must also support appropriate mechanics, degradation, maturation, and integration with the host tissue.
Construct studies can reveal how scaffold architecture, cell behavior, transport, material properties, and controlled cues affect tissue maturation. They also help researchers assess extracellular matrix deposition and the likelihood of integration with host tissue. These observations inform design decisions before regenerative medicine applications, helping connect laboratory-engineered tissues with the requirements of functional implants.