/
/
Engineering Fibrin-based Tissue Constructs from Myofibroblasts and Application of Constraints and Strain to Induce Cell and Collagen Reorganization
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 01:40Culture of Human Vena Saphena Cells
- 03:18Engineering of Fibrin-based Tissue Constructs
- 07:23Applying Strain and Inducing Changes in Stain and Constraints
- 09:25Visualizing Cells and Collagen
- 10:43Results: Changes in Cellular and Matrix Alignment Due to Controlled Strain
- 11:55Conclusion
This model system starts from a myofibroblast-populated fibrin gel that can be used to study endogenous collagen (re)organization real-time in a nondestructive manner. The model system is very tunable, as it can be used with different cell sources, medium additives, and can be adapted easily to specific needs.
