Beta-lactamase Biosensor
Beta-lactamase Biosensor - Related Videos
08:06
The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions
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Cited by 1 •
2018
In this work, we report a new method to study protein-protein interactions using a conductimetric biosensor based on the hybrid β-lactamase technology. This method relies on release of protons upon hydrolysis of β-lactams.
04:43
β-Lactamase-Based Conductimetric Biosensor Assay for Protein-Protein Interactions
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2025
This video demonstrates a conductimetric biosensor assay to study the interaction of an antibody with a target antigen immobilized on a transducer chip. The biosensor is engineered to have a bifunctional chimeric protein — a β-lactamase enzyme with a functional catalytic site fused to a nanobody for binding to the immobilized target antigen. Upon binding, the catalytic hydrolysis of an antibiotic substrate by the β-lactamase enzyme generates an electrical signal — confirming the...
12:23
Analysis of Yersinia enterocolitica Effector Translocation into Host Cells Using Beta-lactamase Effector Fusions
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Cited by 5 •
2015
Effector translocation into host cells via a type III secretion system is a common virulence strategy among gram-negative bacteria. A beta-lactamase effector fusion based assay for quantitative analysis of translocation was applied. In Yersinia infected cells, conversion of a FRET reporter by the beta-lactamase is monitored using laser scanning microscopy.
01:25
Beta
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2024
Beta is a crucial metric in finance used to assess an investment's risk and expected return relative to the overall market. It is calculated as the slope of the line in a scatterplot that compares the returns of an individual stock to the returns of the market benchmark, typically represented by a broad market index. Beta quantifies how much a stock's price moves in response to market movements, providing insight into its volatility and systematic risk. A beta of one indicates that the stock's...
14:04
Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria
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Cited by 17 •
2013
Lytic phage biosensors and antibody beads are able to discriminate between methicillin resistant (MRSA) and sensitive staphylococcus bacteria. The phages were immobilized by a Langmuir-Blodgett method onto a surface of a quartz crystal microbalance sensor and worked as broad range staphylococcus probes. Antibody beads recognize MRSA.
FAQs
The central reaction is enzymatic hydrolysis of a beta-lactam substrate. When beta-lactamase acts on that substrate, the resulting chemical change is coupled to a measurable physical response, such as altered color, fluorescence, or electrical behavior. The sensor therefore translates enzyme activity into an analytical readout that can be associated with beta-lactam resistance.
A Beta-lactamase Biosensor can convert substrate hydrolysis into several signal types, including colorimetric, fluorescent, electrical, or other physical changes. These formats represent different ways to observe the same enzyme-driven event. The available readout determines how the reaction is monitored and supports targeted measurement of beta-lactamase activity in a sample.
Signal intensity provides a measurable indication of the enzyme reaction occurring at the sensor. Because the response is linked to beta-lactamase activity, differences in intensity can support comparisons among samples during screening or susceptibility assessment. This relationship is central to using the device as an analytical tool rather than simply observing substrate exposure.
A basic workflow places a sample in contact with a beta-lactam substrate incorporated into or used with the sensor, allows the enzyme reaction to occur, and records the resulting physical signal. The measured response is then interpreted as evidence of beta-lactamase activity. This sequence connects sample analysis directly to the hydrolysis reaction.
Researchers can apply the method when they need to screen bacteria for beta-lactamase activity or support antibiotic susceptibility assessment. Its targeted response helps identify enzyme activity associated with resistance to beta-lactam antibiotics. In microbiology and antibiotic-resistance research, the resulting measurement can contribute to evaluating resistant bacterial samples.
These sensors can be used to monitor beta-lactamase contamination in biological or environmental samples, extending their value beyond bacterial screening. They also have relevance to clinical diagnostics, where detecting resistance-associated enzyme activity can inform analysis of bacterial samples. Their sensitivity and targeted measurement make them useful across microbiology, diagnostics, and resistance research.