Crispr Base Editing
Crispr Base Editing - Related Videos
02:58
CRISPR-Mediated Base Editing Tools: A Genome Editing Technique to Induce Targeted Base Substitution
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2025
This video explains the concept of CRISPR-mediated cytosine base editors for inducing targeted nucleotide substitution.
05:12
All-in-One CRISPR Genome Editing: A Method for Homology Directed Repair-Based Gene Knock-In in Cultured Cells Using CRISPR-Cas9 System
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2025
In this video, we demonstrate all-in-one CRISPR-Cas9 based genome editing in cultured cells where Cas9 and sgRNA are provided as a single plasmid construct to the cells. The CRISPR-Cas9 system and desired gene to be inserted was introduced in cells through electroporation technique to facilitate successful gene editing.
11:37
QTL Mapping and CRISPR/Cas9 Editing to Identify a Drug Resistance Gene in Toxoplasma gondii
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Cited by 5 •
2017
Details are presented on how QTL mapping with a whole genome sequence based genetic map can be used to identify a drug resistance gene in Toxoplasma gondii and how this can be verified with the CRISPR/Cas9 system that efficiently edits a genomic target, in this case the drug resistance gene.
04:22
A Technique for Gene Editing in Natural Killer Cells Using CRISPR Cas9
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2025
This video demonstrates a technique for Cas9 ribonucleoprotein-mediated genetic modification of primary natural killer (NK) cells. A Cas9 ribonucleoprotein, consisting of a Cas9 endonuclease bound to a guide RNA (gRNA) formed by base pairing a CRISPR RNA (crRNA) and a trans-activating crRNA (tracrRNA), is introduced into primary natural killer cells via electroporation. The ribonucleoprotein targets and cleaves the host DNA at the target site, leading to gene knockout via modification of the...
08:31
Genome Editing
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2023
A well-established technique for modifying specific sequences in the genome is gene targeting by homologous recombination, but this method can be laborious and only works in certain organisms. Recent advances have led to the development of “genome editing”, which works by inducing double-strand breaks in DNA using engineered nuclease enzymes guided to target genomic sites by either proteins or RNAs that recognize specific sequences. When a cell attempts to repair this damage, mutations can be...
FAQs
The deaminase performs the chemical step that changes one exposed DNA base into another. Its activity is directed to the sequence selected by the guide RNA and modified Cas protein, allowing the system to alter a specific base rather than broadly modifying the surrounding DNA. This chemical conversion is central to the method’s precision and lower-disruption editing strategy.
The Cas nickase makes a single-strand nick that helps the cell resolve the edited DNA strand after deaminase activity. Because this differs from cutting both DNA strands, the editing process avoids the double-strand break used in conventional CRISPR approaches. The nickase therefore supports installation of the intended base conversion while limiting the type of DNA disruption associated with broader cutting methods.
The available conversion depends on the editor’s biochemical configuration, particularly the attached deaminase. In the described systems, one editor supports adenine-to-guanine conversion, whereas another supports cytosine-to-thymine conversion. Choosing between them must therefore match the base substitution being investigated, which is especially important when modeling or studying a specific disease-associated point mutation.
Conventional CRISPR approaches use double-strand DNA breaks, whereas base editing changes an individual base through targeted chemical conversion and strand resolution. This distinction gives base editing a lower-disruption strategy for selected point changes. As a result, it is particularly suited to experiments requiring focused alteration of a nucleotide rather than a broader editing event.
A typical workflow begins by identifying the DNA sequence and base substitution of interest, then selecting a compatible guide RNA and editor. The guide RNA directs the modified Cas protein to the target, while the attached deaminase performs the appropriate conversion. The resulting edited sequence can then be examined in the context of gene function, variant behavior, or mutation correction.
Researchers can use the method to investigate how individual genetic changes affect gene function, examine disease-associated variants, or study potential correction of point mutations. These applications benefit from the ability to focus on a single-base change without the double-strand breaks used in conventional CRISPR approaches. The technique therefore connects targeted molecular editing with functional and disease-oriented biological studies.
The approach can generate a defined adenine-to-guanine or cytosine-to-thymine substitution at a selected sequence, depending on the editor used. Such targeted changes allow researchers to test the consequences of an individual nucleotide variant in a biological system. In disease-related research, the same capability supports evaluation of whether correcting a point mutation could influence the associated biological function.