3d In Vitro Models
3d In Vitro Models - Related Videos
08:25
Advanced 3D Liver Models for In vitro Genotoxicity Testing Following Long-Term Nanomaterial Exposure
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Cited by 21 •
2020
This procedure was established to be used for developing advanced 3D hepatic cultures in vitro, which can provide a more physiologically relevant assessment of the genotoxic hazards associated with nanomaterial exposures over both an acute or long-term, repeated dose regimes.
09:14
Improving 2D and 3D Skin In Vitro Models Using Macromolecular Crowding
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Cited by 14 •
2016
We present a protocol to obtain cell-derived matrices rich in extracellular matrix proteins, using macromolecular crowders (MMC). In addition, we present a protocol which incorporates MMC in 3D organotypic skin co-culture generation, which reduces culture time while maintaining maturity of construct.
09:47
Interfacing 3D Engineered Neuronal Cultures to Micro-Electrode Arrays: An Innovative In Vitro Experimental Model
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Cited by 16 •
2015
In this work, a novel experimental model in which 3D neuronal cultures are coupled to planar Micro-Electrode Arrays (MEAs) is presented. 3D networks are built by seeding neurons in a scaffold made up of glass microbeads on which neurons grow and form interconnected 3D structures.
11:12
Tissue Engineering of a Human 3D in vitro Tumor Test System
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Cited by 34 •
2013
Methods to create human 3D tumor tissues as test systems are described. These technologies are based on a decellularized Biological Vascularized Scaffold (BioVaSc), primary human cells and a tumor cell line, which can be cultured under static as well as under dynamic conditions in a flow bioreactor.
04:43
Esophageal 3D Raft Culture Model: An In Vitro Culture Technique to Generate Human Esophageal Mucosa Model
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2023
This video describes an in vitro culture technique to generate human organotypic esophageal constructs by co-culturing primary human esophageal fibroblast and squamous epithelial cells within a decellularized porcine scaffold. These 3D culture models mimic the function and physiology of their in vivo tissue counterparts.
FAQs
Scaffolds and hydrogels provide a surrounding matrix that helps position cells and regulate cell-matrix interactions. This spatial environment can influence how cells organize, communicate, and perform tissue-related functions. Because matrix conditions affect cellular behavior, selecting an appropriate scaffold or hydrogel is important when the goal is to reproduce particular aspects of tissue architecture or function.
Spatial organization determines which cells interact and where those interactions occur, while chemical gradients expose different regions of a model to different local conditions. Together, these features can produce biological behavior that is not captured by uniformly arranged cells. They therefore help researchers examine how tissue structure and local signals influence development, disease mechanisms, or treatment responses.
Three-dimensional systems add tissue-like architecture, cell-cell interactions, and cell-matrix relationships that conventional two-dimensional cultures simplify. At the same time, they provide a laboratory-based intermediate between simplified culture and animal models. This position can improve biological insight and support research strategies that may reduce reliance on animal studies while retaining controlled experimental conditions.
The chosen format depends on how researchers need cells to organize and which aspects of tissue architecture or function they want to study. Organoids, spheroids, and engineered tissues represent different model types within the broader 3D in vitro approach. Their usefulness is judged by how well the resulting organization and behavior match the biological question under investigation.
Design begins by defining the tissue behavior or biological question the model should represent. Researchers then select a way to support cellular organization, such as an extracellular matrix scaffold, hydrogel, or self-assembled structure. The resulting system can be evaluated through its architecture, cell-cell and cell-matrix interactions, chemical gradients, and relevant functional behavior.
They are useful when treatment effects may depend on tissue organization, cell interactions, or local chemical conditions. A three-dimensional system can be used to examine whether a candidate treatment produces the intended response and whether it causes harmful effects in a more physiologically relevant setting than conventional two-dimensional culture. These outcomes can strengthen interpretation of drug studies.
By recreating aspects of tissue architecture and function, 3D systems allow researchers to examine how cells organize and interact during biological processes. The same features can help investigate how altered organization or signaling contributes to disease mechanisms. This makes the models relevant for connecting cellular behavior with tissue-level changes in developmental and disease research.
These systems can provide a platform for examining biological responses in model conditions relevant to an individual research or treatment context. Their ability to represent tissue organization and function supports evaluation of how disease-related biology or treatments may behave in a more physiologically relevant setting. Consequently, they can contribute to personalized medicine alongside drug and disease studies.