Adenosine Triphosphate

Adenosine triphosphate (ATP) is the cell’s primary energy-transfer molecule, linking energy-releasing reactions to the work required for life. Its adenine, ribose, and three phosphate groups enable ATP hydrolysis to ADP and inorganic phosphate, a reaction that can drive biosynthesis, active transport, and mechanical movement; cells regenerate ATP by adding phosphate to ADP during processes such as cellular respiration and photosynthesis. ATP also participates in phosphorylation, modifying proteins and regulating metabolic pathways. Understanding ATP clarifies how cells store, distribute, and use energy, making it central to studies of metabolism, physiology, and disease.

Adenosine Triphosphate - Related Videos

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JoVE EoE - Leukemia

Extracellular Purine Metabolism: An HPLC-based Assay to Measure Adenosine Generation and Metabolism in Leukemic Cells

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2023

This video explains a quantitative reverse phase high-performance liquid chromatography (RP-HPLC) method to measure metabolism of adenosine and associated purines generated by consumption of AMP in extracellular environment of leukemic cancer cells. The protocol describes a sensitive, specific, reliable and reproducible RP-HPLC assay developed and validated for the quantification of extracellular purine nucleotides and nucleosides produced by purified chronic lymphocytic leukemia (CLL) cells...

Nucleoside Triphosphates - From Synthesis to Biochemical Characterization

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Cited by 4 •

2014

The protocol described herein aims to explain and abridge the numerous obstacles in the way of the intricate route leading to modified nucleoside triphosphates. Consequently, this protocol facilitates both the synthesis of these activated building-blocks and their availability for practical applications.

Determining the Serum Stability of Human Adenosine Deaminase 1 Enzyme

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2024

In this article, we detail methods to characterize an enzyme's ability to retain function when incubated at 37 °C in human serum, a pharmacological property referred to as its serum stability. This ability may be a key factor in predicting an enzyme's pharmacokinetic profile and its suitability for therapeutic use.

Testing Acetylcholine Followed by Adenosine for Invasive Diagnosis of Coronary Vasomotor Disorders

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Cited by 6 •

2021

Coronary vasomotion disorders represent frequent functional causes of angina in patients with unobstructed coronaries. The underlying mechanism of angina (endotype) in these patients can be determined by a comprehensive invasive diagnostic procedure based on acetylcholine provocation testing followed by Doppler-derived assessment of the coronary flow reserve and microvascular resistance.

Measuring Odorant Receptor Activation using a Real-Time Cyclic Adenosine Monophosphate Assay

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2025

This video demonstrates the protocol for measuring odorant receptor activation in transfected cells using a cyclic adenosine monophosphate (cAMP) biosensor and luminescence detection. The method is applied to study receptor-ligand interactions and signaling pathways in olfactory neurons.

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