Antisense Rna Probes
Antisense Rna Probes - Related Videos
03:26
Probe Hybridization and Signal Amplification in RNA In Situ Hybridization: A Technique for Detecting Specific RNA Sequences in Tissue Sections
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2023
This video describes a sequential hybridization strategy that involves hybridizing mRNA probes to specific target mRNA. Following probe hybridization, signal amplification is performed to enhance resolution via reduction of signal-to-noise ratio during RNA-CISH of histological samples.
06:21
Depletion of Ribosomal RNA for Mosquito Gut Metagenomic RNA-seq
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Cited by 20 •
2013
A ribosomal RNA (rRNA) depletion protocol was developed to enrich messenger RNA (mRNA) for RNA-seq of the mosquito gut metatranscriptome. Sample specific rRNA probes, which were used to remove rRNA via subtraction, were created from the mosquito and its gut microbes. Performance of the protocol can result in the removal of approximately 90-99% of rRNA.
06:08
In Situ Hybridization Using Oligonucleotide Probes to Study RNA Expression in Rat Brain Sections
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2025
This video demonstrates the detection of serotonin receptor RNA synthesis using oligonucleotide probes in rat brain tissue affected by serotonin syndrome. The protocol includes tissue pretreatment, probe hybridization, and enzymatic detection to visualize and quantify serotonin receptor RNA expression under a microscope.
11:33
Microinjection of mRNA and Morpholino Antisense Oligonucleotides in Zebrafish Embryos.
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Cited by 86 •
2009
Microinjection is a well-established and effective method for introducing foreign substances into fertilized zebrafish embryos. Here, we demonstrate a robust microinjection technique for performing mRNA overexpression, and morpholino oligonucleotide gene knockdown studies in zebrafish.
10:30
Multi-exon Skipping Using Cocktail Antisense Oligonucleotides in the Canine X-linked Muscular Dystrophy
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Cited by 23 •
2016
Exon skipping is currently a most promising therapeutic option for Duchenne muscular dystrophy (DMD). To expand the applicability for DMD patients and to optimize the stability/function of the resulting truncated dystrophin proteins, a multi-exon skipping approach using cocktail antisense oligonucleotides was developed and we demonstrated systemic dystrophin rescue in a dog model.
FAQs
Complementary base pairing gives an antisense RNA probe sequence-level selectivity: only an RNA region with the matching sequence can form the intended probe-target complex. This selectivity allows investigators to associate a detected signal with a particular transcript rather than with RNA distribution in general, supporting transcript-specific analysis in cells and tissues.
Label choice determines how the hybridization result is recorded. Fluorescent labels make the probe-target complex visible, radioactive labels provide a measurable signal, and enzymatic labels also enable detection through an observable or measurable output. These alternatives let the same targeting strategy support different ways of examining transcripts in biological samples.
Controlled hybridization conditions are essential because the probe must encounter and pair with its complementary target RNA in a reproducible setting. Conditions surrounding this step influence whether the resulting complex can be detected and interpreted. Careful control therefore connects the molecular pairing event to reliable measurements of transcript presence or distribution.
An antisense RNA probe experiment begins by selecting a sequence complementary to the transcript of interest, followed by hybridization with the sample under controlled conditions. Researchers then detect the attached fluorescent, radioactive, or enzymatic label. In situ hybridization applies this workflow to cells or tissues, preserving spatial information about where the transcript occurs.
When the goal is to map RNA within a specimen, in situ hybridization is especially informative because detection remains tied to cellular or tissue location. The resulting pattern can reveal transcript localization and cell-specific expression patterns. This makes the approach useful for examining how RNA distribution relates to cellular organization and function.
In developmental or disease studies, probe-based transcript detection helps compare RNA distribution across biological contexts. Changes in RNA distribution or detection patterns can be examined alongside questions about gene regulation and cellular function. The method therefore links transcript-level observations with tissue organization, developmental processes, or disease-associated differences.