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Biology
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Depletion of Ribosomal RNA for Mosquito Gut Metagenomic RNA-seq
 

Depletion of Ribosomal RNA for Mosquito Gut Metagenomic RNA-seq

Article DOI: 10.3791/50093
April 7th, 2013

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Summary April 7th, 2013

A ribosomal RNA (rRNA) depletion protocol was developed to enrich messenger RNA (mRNA) for RNA-seq of the mosquito gut metatranscriptome. Sample specific rRNA probes, which were used to remove rRNA via subtraction, were created from the mosquito and its gut microbes. Performance of the protocol can result in the removal of approximately 90-99% of rRNA.

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