Aortic Ring Culture
Aortic Ring Culture - Related Videos
08:20
Isolation and Primary Culture of Mouse Aortic Endothelial Cells
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Cited by 75 •
2016
The vascular endothelial cells play a significant role in many important cardiovascular disorders. This article describes a simple method to isolate and expand endothelial cells from the mouse aorta without using any special equipment. Our protocol provides an effective means of identifying mechanisms in endothelial cell physiopathology.
09:12
Aortic Ring Assay
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Cited by 42 •
2009
Angiogenesis, the sprouting of blood vessels from pre-existing vasculature, is associated with both natural and pathological processes. Here we demonstrate an aortic ring assay that allows angiogenic potentiators and inhibitors to be directly added to aortic rings in culture. Sprouting and neovessel outgrowth can be determined by inspecting the aortic rings over a period of 6-12 days.
12:17
Full-root Aortic Valve Replacement by Stentless Aortic Xenografts in Patients with Small Aortic Roots
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Cited by 4 •
2017
Full-root aortic valve replacement by stentless aortic xenograft is a viable option in patients with small aortic roots. We describe, a technique for the full-root implantation of stentless aortic xenografts, with emphasis on the management of the proximal suture line and coronary anastomoses, and discuss its limitations and alternative options.
10:04
The Aortic Ring Co-culture Assay: A Convenient Tool to Assess the Angiogenic Potential of Mesenchymal Stromal Cells In Vitro
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Cited by 12 •
2017
Here, we present a novel application of the aortic ring assay where prelabelled mesenchymal cells are co-cultured with rat aorta-derived endothelial networks. This novel method allows visualization of Mesenchymal Stromal Cells (MSCs) homing and integration with endothelial networks, quantification of network properties, and evaluation of MSC immunophenotypes and gene expression.
04:51
Isolation of Mouse Valve Interstitial Cells: An Enzymatic Method to Isolate and Culture VICs from Mouse Aortic Valve
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2025
In this video, we demonstrate the isolation of aortic valve interstitial cells from the heart of an adult mouse using a two-step collagenase procedure and their subsequent culture in low volume of growth media.
FAQs
The cut edges expose vessel-wall cells to the surrounding three-dimensional matrix, allowing endothelial cells, smooth muscle cells, and supporting vascular cells to migrate outward. These cells can form microvascular sprouts that extend from the ring and provide a visible, measurable response. The sprouting pattern therefore reflects coordinated activity among several vascular cell populations rather than endothelial behavior alone.
The matrix provides a physical environment in which cells can migrate away from the vessel segment and organize into sprouts. This setting preserves spatial relationships between the ring and emerging microvessels, enabling researchers to observe vascular outgrowth under controlled laboratory conditions. It also helps the assay model tissue-level behavior more closely than a simplified culture containing isolated cells.
Aortic ring culture retains multiple interacting cell types from the vessel wall, including endothelial, smooth muscle, and supporting vascular cells. Simplified cell-based assays can focus more narrowly on a single population or response. By preserving multicellular interactions, the ring model can reveal how combined vascular behavior contributes to angiogenesis and remodeling, while complementing rather than replacing reductionist experiments.
Researchers maintain cross-sectional aortic segments, embed the rings in a three-dimensional extracellular matrix, and allow cells to migrate from the cut edges. During the culture period, microvascular sprouts may develop outward from the tissue. Investigators then use the resulting sprouting response as a measurable readout for vascular behavior under the selected laboratory conditions.
The assay can be used to assess both pro-angiogenic signals, which promote microvascular sprouting, and anti-angiogenic signals, which limit that response. Comparing sprouting under different controlled conditions helps investigators examine how vascular tissues respond to these opposing influences. This makes the model useful for studying regulation of angiogenesis without reducing the system to one isolated cell type.
Researchers apply the model to investigate angiogenesis, vascular remodeling, and vascular disease-related behavior, as well as to evaluate potential therapeutics. Its multicellular tissue context can show how candidate treatments influence coordinated vessel outgrowth rather than only a response from isolated cells. The assay therefore provides a bridge between simplified cell studies and more integrated investigations of vascular function.