Aortic Tissue Imaging
Aortic Tissue Imaging - Related Videos
09:55
Three-Dimensional Imaging of Aortic Tissues in Atherosclerosis
0 Views •
2024
Here, we present optimized tissue-clearing protocols to image the murine aorta in three dimensions (3D). We delineate state-of-the-art procedures for immunostaining, optical clearing, and imaging with the intent to define the anatomical proximity of the peripheral nervous system with atherosclerotic plaques and the adventitia in atherosclerosis.
10:12
Near-infrared Fluorescence Imaging of Abdominal Aortic Aneurysms
0 Views •
2023
Source: Arvin H. Soepriatna1, Kelsey A. Bullens2, and Craig J. Goergen1 1 Weldon School of Biomedical Engineering, Purdue University, West Lafayette, Indiana 2 Department of Biochemistry, Purdue University, West Lafayette, Indiana Near-infrared fluorescence (NIRF) imaging is an exciting optical technique that utilizes fluorescent probes to visualize complex biomolecular assemblies in tissues. NIRF imaging has many advantages over conventional imaging methods for noninvasive imaging of diseases.
09:12
Aortic Ring Assay
0 Views •
Cited by 42 •
2009
Angiogenesis, the sprouting of blood vessels from pre-existing vasculature, is associated with both natural and pathological processes. Here we demonstrate an aortic ring assay that allows angiogenic potentiators and inhibitors to be directly added to aortic rings in culture. Sprouting and neovessel outgrowth can be determined by inspecting the aortic rings over a period of 6-12 days.
08:43
Calcification of Vascular Smooth Muscle Cells and Imaging of Aortic Calcification and Inflammation
0 Views •
Cited by 28 •
2016
Vascular calcification is an important predictor of and contributor to human cardiovascular disease. This protocol describes methods for inducing calcification of cultured primary vascular smooth muscle cells and for quantifying calcification and macrophage burden in animal aortas using near-infrared fluorescence imaging.
12:17
Full-root Aortic Valve Replacement by Stentless Aortic Xenografts in Patients with Small Aortic Roots
0 Views •
Cited by 4 •
2017
Full-root aortic valve replacement by stentless aortic xenograft is a viable option in patients with small aortic roots. We describe, a technique for the full-root implantation of stentless aortic xenografts, with emphasis on the management of the proximal suture line and coronary anastomoses, and discuss its limitations and alternative options.
FAQs
Each modality converts a different physical or molecular signal into visual information. Ultrasound can support assessment of wall thickness and blood flow, whereas X-rays and magnetic fields provide other routes to structural visualization; fluorescent labels add molecular features. Choosing among these readouts determines whether an experiment emphasizes tissue organization, hemodynamics, or biological activity.
Structural measurements show how the vessel is organized, while functional measurements capture processes such as blood flow. Interpreting both together can connect visible changes in the aortic wall with hemodynamic conditions. This combined view is valuable because pathology may involve altered structure and altered function, rather than an isolated change in only one measurement.
Aortic tissue imaging can place changes in the extracellular matrix, smooth muscle cells, and inflammation alongside measurable vessel features. Relating these biological components to wall organization, thickness, or blood flow helps researchers examine how vascular remodeling and injury responses accompany disease progression. The approach therefore links cellular and tissue-level observations rather than treating pathology as an image alone.
These approaches differ mainly in the signals they use and the information they make visible. Ultrasound can reveal blood flow or wall thickness, while other methods support structural visualization, and fluorescent labels can expose molecular features. The most informative choice depends on whether the investigation prioritizes tissue organization, hemodynamics, molecular activity, or a combination of these outcomes.
Researchers can begin by selecting the imaging approach that matches the biological question, such as assessing wall thickness, blood flow, tissue organization, or molecular features. They then acquire the relevant signal, convert it into an image, and analyze the resulting structural or functional measurements. Comparing those measurements with vascular pathology supports interpretation of disease-related changes.
The approach supports studies of atherosclerosis, aneurysm formation, vascular remodeling, and responses to injury or treatment. Researchers can use the resulting measurements to examine how extracellular matrix changes, smooth muscle cells, inflammation, and hemodynamics relate to disease progression. In broader research contexts, these findings can strengthen experimental models and inform diagnostic or therapeutic strategy development.