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1Anesthesia Center for Critical Care Research of the Department of Anesthesia, Critical Care, and Pain Medicine,Massachusetts General Hospital, 2Cardiovascular Research Center and Cardiology Division of the Department of Medicine,Massachusetts General Hospital, 3Cardiovascular Division,Brigham and Women’s Hospital, 4Harvard Medical School, 5Department of Anesthesiology,Uniklinik RWTH Aachen, RWTH Aachen University, 6Center for Immunology and Inflammatory Diseases and the Division of Rheumatology, Allergy, and Immunology of the Department of Medicine,Massachusetts General Hospital
Chapters
- 00:05Title
- 00:58Tail Vein Injection
- 01:35Mouse Dissection
- 03:39Isolation of Primary Murine Aortic VSMCs
- 05:21Assessing VSMC Calcification Using the Von Kossa Staining Method
- 06:43Results: MGP Deficiency Results in Vascular Calcification in the Absence of Vascular Inflammation and MGP Protects Cultured VSMCs from Calcification
- 08:03Conclusion
Vascular calcification is an important predictor of and contributor to human cardiovascular disease. This protocol describes methods for inducing calcification of cultured primary vascular smooth muscle cells and for quantifying calcification and macrophage burden in animal aortas using near-infrared fluorescence imaging.
