Calcification of Vascular Smooth Muscle Cells and Imaging of Aortic Calcification and Inflammation

Caitlin O'Rourke; Georgia Shelton; Joshua D. Hutcheson; Megan F. Burke; Trejeeve Martyn; Timothy E. Thayer; Hannah R. Shakartzi; Mary D. Buswell; Robert E. Tainsh; Binglan Yu; Aranya Bagchi; David K. Rhee; Connie Wu; Matthias Derwall; Emmanuel S. Buys; Paul B. Yu; Kenneth D. Bloch; Elena Aikawa; Donald B. Bloch; Rajeev Malhotra

doi:10.3791/54017

Journal

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Medicine

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Calcification of Vascular Smooth Muscle Cells and Imaging of Aortic Calcification and Inflammation

JoVE Journal
Medicine

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JoVE Journal Medicine

Calcification of Vascular Smooth Muscle Cells and Imaging of Aortic Calcification and Inflammation

19,490 Views

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08:43 min

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May 31, 2016

DOI:

10.3791/54017-v

DOI:

10.3791/54017-v

•

08:43 min

May 31, 2016

•

19476 Views

¹Anesthesia Center for Critical Care Research of the Department of Anesthesia, Critical Care, and Pain Medicine,Massachusetts General Hospital ²Cardiovascular Research Center and Cardiology Division of the Department of Medicine,Massachusetts General Hospital ³Cardiovascular Division,Brigham and Women’s Hospital ⁴Harvard Medical School ⁵Department of Anesthesiology,Uniklinik RWTH Aachen, RWTH Aachen University ⁶Center for Immunology and Inflammatory Diseases and the Division of Rheumatology, Allergy, and Immunology of the Department of Medicine,Massachusetts General Hospital

Summary

Vascular calcification is an important predictor of and contributor to human cardiovascular disease. This protocol describes methods for inducing calcification of cultured primary vascular smooth muscle cells and for quantifying calcification and macrophage burden in animal aortas using near-infrared fluorescence imaging.