Arabidopsis Seedlings
Arabidopsis Seedlings - Related Videos
11:16
Isolation of Protoplasts from Tissues of 14-day-old Seedlings of Arabidopsis thaliana
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Cited by 59 •
2009
This video shows a procedure for isolating intact protoplasts from tissues of 14-day-old seedlings of Arabidopsis. Given that the isolated protoplasts remain intact for at least 96h and are isolated from seedlings instead of one-month-old mature plants, this procedure expedites assays requiring intact protoplasts.
09:23
Imaging the Root Hair Morphology of Arabidopsis Seedlings in a Two-layer Microfluidic Platform
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Cited by 11 •
2017
This article demonstrates how to culture Arabidopsis thaliana seedlings in a two-layer microfluidic platform that confines the main root and root hairs to a single optical plane. This platform can be used for real-time optical imaging of fine root morphology as well as for high-resolution imaging by other means.
09:23
Lateral Root Inducible System in Arabidopsis and Maize
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Cited by 8 •
2016
The Lateral Root Inducible System (LRIS) allows for synchronous induction of lateral roots and is presented for Arabidopsis thaliana and maize.
04:11
Pattern-Triggered Oxidative Burst and Seedling Growth Inhibition Assays in Arabidopsis thaliana
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Cited by 22 •
2019
This paper describes two methods for quantifying defense responses in Arabidopsis thaliana following exposure to immune elicitors: the transient oxidative burst, and the inhibition of seedling growth.
04:32
An Induction System for Clustered Stomata by Sugar Solution Immersion Treatment in Arabidopsis thaliana Seedlings
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Cited by 3 •
2019
The goal of this protocol is to demonstrate how to induce clustered stomata in cotyledons of Arabidopsis thaliana seedlings by immersion treatment with a sugar-containing medium solution and how to observe intracellular structures such as chloroplasts and microtubules in the clustered guard cells using confocal laser microscopy.
FAQs
Light and plant hormones act as coordinating signals during early Arabidopsis development. Auxin, a plant hormone, helps regulate developmental changes while light provides environmental information that influences the seedling’s form and growth. By controlling these inputs, researchers can investigate how external conditions and internal signaling jointly shape root, hypocotyl, and cotyledon development.
Cell division and cell elongation contribute different but coordinated aspects of seedling growth. Following these processes helps connect changes in cellular behavior to formation of the primary root, hypocotyl, and cotyledons. This makes the system useful for asking whether a gene, hormone, or environmental cue affects organ development, rather than only describing the final plant structure.
Researchers can investigate gravitropism by examining how seedling growth responds under controlled conditions and by using imaging to document developmental changes. Mutants provide genetic comparisons that can help link altered growth patterns to gene function. This combination connects an observable growth response with the developmental regulation of roots and other seedling tissues.
A practical observation sequence starts with imbibition and germination, then tracks radicle emergence and development of the primary root, hypocotyl, and cotyledons. Researchers can maintain seedlings under controlled growth conditions and image them at selected stages. This workflow provides a time-resolved view of early development for comparing genotypes, treatments, or environmental conditions.
Imaging, mutants, and controlled growth conditions provide complementary types of evidence. Imaging records developmental features and growth changes, mutants help test gene function, and controlled conditions make environmental influences easier to examine. Used together, these approaches allow researchers to connect visible seedling phenotypes with genetic factors and regulated developmental processes.
Studies of Arabidopsis seedlings address photomorphogenesis, root development, stress responses, gravitropism, and gene function. Their rapid development and experimental accessibility allow researchers to examine these processes during a clearly observable stage. Results can clarify fundamental mechanisms of plant development and provide context for research in agriculture and environmental biology.