Arabidopsis Thaliana
Arabidopsis Thaliana - Related Videos
02:32
Monitoring Bacterial Colonization of Arabidopsis thaliana Roots in a Soilless System
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2026
Source: Harris, S. L., et al. Monitoring Bacterial Colonization and Maintenance on Arabidopsis thaliana Roots in a Floating Hydroponic System. J. Vis. Exp. (2019)This video demonstrates a protocol for monitoring the colonization of root-associated bacteria on Arabidopsis thaliana seedlings grown in a soilless system. Following colonization, bacteria are dislodged by sonication, then diluted, plated, and incubated to form colonies that reflect bacterial attachment and viability.
04:29
Assessing Pseudomonas syringae Infection Load in Arabidopsis thaliana Leaves
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2026
Source: Liu, X., et al. Bacterial Leaf Infiltration Assay for Fine Characterization of Plant Defense Responses using the Arabidopsis thaliana-Pseudomonas syringae Pathosystem. J. Vis. Exp. (2015).This video demonstrates a method to assess the bacterial load in Arabidopsis thaliana leaves infected with Pseudomonas syringae. Leaf discs from infected leaves are homogenized, serially diluted, and plated onto nutrient-rich agar to promote bacterial colony formation. Colony counts are then used to...
11:16
Isolation of Protoplasts from Tissues of 14-day-old Seedlings of Arabidopsis thaliana
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Cited by 59 •
2009
This video shows a procedure for isolating intact protoplasts from tissues of 14-day-old seedlings of Arabidopsis. Given that the isolated protoplasts remain intact for at least 96h and are isolated from seedlings instead of one-month-old mature plants, this procedure expedites assays requiring intact protoplasts.
09:54
Isolation and Respiratory Measurements of Mitochondria from Arabidopsis thaliana
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Cited by 15 •
2018
As mitochondria are only a small percentage of the plant cell, they need to be purified for a range of studies. Mitochondria can be isolated from a variety of plant organs by homogenization, followed by differential and density gradient centrifugation to obtain a highly purified mitochondrial fraction.
10:39
Histochemical Staining of Arabidopsis thaliana Secondary Cell Wall Elements
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Cited by 250 •
2014
Plant cell wall composition varies between tissue types and can include lignin, cellulose, hemicelluloses, and pectin. Various staining techniques have been developed to visualize differences at the cell-type level. This paper is a compilation of commonly used cell wall staining techniques.
FAQs
Researchers alter genes through mutations or by introducing transgenes, then examine changes in development, physiology, reproduction, or environmental responses. A visible or measurable difference after genetic alteration can connect a gene to a biological process. This strategy allows experiments to move from a genetic change to an observed phenotype and supports functional studies across multiple aspects of plant biology.
Light, temperature, and drought are important conditions for testing how plants respond to their surroundings. Researchers compare developmental or physiological effects under these conditions to investigate environmental response pathways. Such experiments can reveal how genetic changes influence the plant’s ability to adjust its growth, reproduction, or other biological functions when external conditions vary.
Research using this plant has clarified mechanisms associated with flowering, hormone signaling, circadian regulation, and plant immunity. These areas connect environmental information and internal regulation with observable biological outcomes. Studying them in a genetically tractable system helps researchers analyze how particular genes contribute to timing, signaling, daily biological rhythms, and defense-related processes.
A common workflow begins by introducing a mutation or transgene and then observing the resulting plant characteristics. Investigators may assess development, physiology, reproduction, or responses to light, temperature, or drought. Linking the genetic manipulation to these observations provides evidence about gene function and creates a basis for comparing related biological processes.
Its genomic resources and standardized laboratory methods support broader studies of gene regulation, evolution, and biotechnology. These resources make it possible to place individual experimental findings within larger questions about how genes are controlled, how biological traits change, and how plant-based research can contribute to technological applications.
Arabidopsis studies can identify biological principles involving flowering, hormone signaling, circadian regulation, immunity, development, and environmental responses. Those principles can guide investigations in crops and other plant species, even when the original experiment focuses on Arabidopsis. The model therefore provides a research context for connecting controlled genetic studies with broader questions in plant biology.