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JoVE Journal
Biochemistry

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Isolation and Respiratory Measurements of Mitochondria from Arabidopsis thaliana
 

Isolation and Respiratory Measurements of Mitochondria from Arabidopsis thaliana

Article DOI: 10.3791/56627
January 5th, 2018

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Summary January 5th, 2018

As mitochondria are only a small percentage of the plant cell, they need to be purified for a range of studies. Mitochondria can be isolated from a variety of plant organs by homogenization, followed by differential and density gradient centrifugation to obtain a highly purified mitochondrial fraction.

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