Bac Clones
Bac Clones - Related Videos
09:55
Molecular Cloning
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2023
Molecular cloning is a set of methods, which are used to insert recombinant DNA into a vector - a carrier of DNA molecules that will replicate recombinant DNA fragments in host organisms. The DNA fragment, which may be a gene, can be isolated from a prokaryotic or eukaryotic specimen. Following isolation of the fragment of interest, or insert, both the vector and insert must be cut with restriction enzymes and purified. The purified pieces are joined together though a technique called...
01:27
Reproductive Cloning
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2019
Reproductive cloning is the process of producing a genetically identical copy—a clone—of an entire organism. While clones can be produced by splitting an early embryo—similar to what happens naturally with identical twins—cloning of adult animals is usually done by a process called somatic cell nuclear transfer (SCNT). Somatic Cell Nuclear Transfer In SCNT, an egg cell is taken from an animal and its nucleus is removed, creating an enucleated egg. Then a somatic cell—any cell that is not a sex...
05:21
Culturing and Harvesting of HSPC Clones: A Method to Obtain the Singly-Sorted HSPC Clones
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2023
This video describes the protocol for isolating HSPCs from the bone marrow and sorting them using a fluorescent activated cell sorting technique, which is a method to obtain singly-sorted HSPCs. We culture these sorted cells to obtain HSPC clones.
06:28
Standardized Modular Assembly of Polycistronic Operons with Modular Cloning (MoClo) using the In-Cloning toolkit
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2025
This article presents a step-by-step protocol demonstrating how Modular Cloning (MoClo) can be adapted for the cloning of polycistronic operons.
08:30
Yeast Transformation and Cloning
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2023
S. cerevisiae are unicellular eukaryotes that are a commonly-used model organism in biological research. In the course of their work, yeast researchers rely upon the fundamental technique of transformation (the uptake of foreign DNA by the cell) to control gene expression, induce genetic deletions, express recombinant proteins, and label subcellular structures. This video provides an overview of how and why yeast transformation is carried out in the lab. The important features of yeast...
FAQs
Low-copy propagation helps preserve long genomic inserts by limiting the replication behavior associated with high-copy plasmid systems. In the BAC strategy, this reduced copy number is linked to fewer rearrangements, so the carried DNA remains more representative of the original genomic region. That stability is especially important when researchers need to maintain and analyze extended, structurally complex loci.
The key distinction is the balance between insert capacity and structural stability. BAC clones carry large genomic fragments while being maintained as low-copy plasmids in Escherichia coli. By contrast, the overview identifies many high-copy systems as more prone to rearrangements. BACs are therefore advantageous when preserving an extended genomic region matters more than maximizing plasmid abundance.
Large inserts allow a single clone to represent a broader portion of a genomic region rather than only a small segment. This makes BAC clones useful for organizing large-scale genetic information and studying complex loci, where relationships among neighboring sequences matter. Their capacity also supports physical mapping, genome sequencing, and gene-isolation efforts.
Researchers first insert genomic DNA into a BAC vector and then introduce the resulting construct into Escherichia coli. The bacterial host propagates the construct as a low-copy plasmid, maintaining the large DNA fragment for subsequent analysis. This workflow connects vector construction, host introduction, and controlled propagation, providing material for genomic libraries and downstream studies.
Collections of BAC clones form genomic libraries that organize many preserved genomic fragments into a resource for analysis. Researchers can use these libraries for physical mapping, genome sequencing, and gene isolation. Because each clone carries a large, stable region, the collection helps arrange and investigate genomic structure at a scale broader than an individual small DNA fragment.
BAC clones are relevant to transgenic research when investigators need access to large genomic regions rather than isolated short sequences. Their capacity and stability support preservation of complex loci before those regions are analyzed or used in a transgenic study. This application shows how BAC resources connect genome organization with experimental investigation of gene-containing DNA.