Bac Reporter Vectors
Bac Reporter Vectors - Related Videos
09:20
Vectors in Multiple Directions
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2023
Source: Nicholas Timmons, Asantha Cooray, PhD, Department of Physics & Astronomy, School of Physical Sciences, University of California, Irvine, CA This experiment demonstrates how vectors add and subtract in multiple directions. The goal will be to analytically calculate the addition or subtraction of multiple vectors and then to experimentally confirm the calculations. A vector is an object with both magnitude and direction. The magnitude of a vector is simply denoted as the length,...
08:46
Production of Lentiviral Vectors for Transducing Cells from the Central Nervous System
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Cited by 44 •
2012
In this protocol we describe production, purification and titration of lentiviral vectors. We provide an example of lentiviral vector-mediated gene delivery in primary cultured neurons and astrocytes. Our methods may also apply to other cell types in vitro and in vivo.
02:08
Transduction of a Humanized Rat Liver Using Adeno-associated Virus Vectors
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2026
Source: Hiller, T., et al. Study of Viral Vectors in a Three-dimensional Liver Model Repopulated with the Human Hepatocellular Carcinoma Cell Line HepG2. J. Vis. Exp., (2016)This video demonstrates adeno-associated virus-mediated gene delivery in a humanized rat liver scaffold. The vector delivers a transgene encoding a fluorescent reporter and gene-silencing RNA into liver cells via receptor-mediated endocytosis. This model supports efficient transduction in a perfused 3D liver tissue system.
10:42
A Protocol for the Production of Integrase-deficient Lentiviral Vectors for CRISPR/Cas9-mediated Gene Knockout in Dividing Cells
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Cited by 21 •
2017
We describe the production strategy of integrase-deficient lentiviral vectors (IDLVs) as vehicles for delivering CRISPR/Cas9 to cells. With an ability to mediate quick and robust gene editing in cells, IDLVs present a safer and equally effective vector platform for gene delivery compared to integrase-competent vectors.
01:24
Data Reporting and Recording
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2023
Reporting and recording are crucial in data documentation. The timely, thorough, and accurate documentation of facts is essential when recording patient data. Failure to record findings during an assessment or interpretation of a problem will result in loss of information and make the patient document unreliable. The reader is left with general impressions if the information is not specific. A recording is documenting data of the individual's health information in a traceable, secure, and...
FAQs
Regulatory fidelity comes from retaining a large genomic region around the target locus. That insert may include endogenous promoters and regulatory sequences located far from the gene, allowing them to influence reporter expression together. Unlike a short promoter fragment, the construct preserves a broader regulatory landscape, which can produce an expression pattern more representative of activity at the native locus.
Recombineering or homologous recombination places the reporter at a selected position within the BAC construct. Once inserted, the reporter remains physically associated with the target genomic region, so regulatory sequences carried in that region can influence its expression. This strategy connects the measurable signal to a defined locus instead of relying only on an isolated, compact promoter sequence.
Compared with compact promoter-only reporters, BAC constructs retain more surrounding genomic context. That context can include distant regulatory elements that a short promoter fragment would omit. The comparison helps researchers determine whether a promoter alone captures the target gene’s regulatory behavior or whether broader locus information is needed to obtain a more faithful expression pattern.
Fluorescent proteins and luciferases are reporter options identified for BAC constructs. Each supplies a detectable output when regulatory sequences drive expression, allowing activity at the target locus to be monitored. The important design principle is that the reporter serves as a readout, while endogenous promoters and distant regulatory elements provide the regulatory control.
Researchers begin with a BAC carrying the genomic region of interest, then use recombineering or homologous recombination to insert a detectable reporter at the target locus. The resulting construct is used to monitor expression in the chosen system, including transgenic cells or animals. The observed reporter pattern reflects control from the genomic sequences retained in the construct.
These vectors are especially useful for mapping when and where a gene is active. Reporter patterns can reveal tissue-specific expression and changes during development, while the same constructs can help assess the contribution of promoters and enhancers. Such applications turn regulatory activity into a detectable signal that can be examined across tissues or developmental stages.
In biology, BAC reporter vectors support transgenic cell or animal models in which regulatory activity can be examined through a reporter rather than inferred from a promoter sequence alone. Their large genomic inserts are particularly relevant when gene control depends on distant elements. These models therefore provide a context-rich view of tissue, developmental, promoter, or enhancer regulation.