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Neuroscience

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Production of Lentiviral Vectors for Transducing Cells from the Central Nervous System
 

Production of Lentiviral Vectors for Transducing Cells from the Central Nervous System

Article DOI: 10.3791/4031
May 24th, 2012

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Summary May 24th, 2012

In this protocol we describe production, purification and titration of lentiviral vectors. We provide an example of lentiviral vector-mediated gene delivery in primary cultured neurons and astrocytes. Our methods may also apply to other cell types in vitro and in vivo.

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