Blood Platelets
Blood Platelets - Related Videos
03:58
Purification and Activation of Platelets from Murine Whole Blood
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2025
In this video, we describe a protocol for purifying platelets from murine whole blood and their subsequent activation with the platelet agonist thrombin. When analyzed with flow cytometry, the purified resting platelets are characterized based on the expression of their cell-surface marker CD41, whereas purified activated platelets co-express both the surface markers CD41 and CD62P.
02:40
Preparation of Platelet-Rich Plasma from Human Peripheral Blood
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2026
Source:Sathish Muthu1,2,31Orthopaedic Research Group, Coimbatore.2Department of Biotechnology, Faculty of Engineering, Karpagam Academy of Higher Education.3Department of Orthopaedics, Government Medical College.This video demonstrates the preparation of high-quality Platelet-Rich Plasma (PRP) by screening for platelet count, collecting and centrifuging blood, and isolating platelets. This PRP is used in therapeutic applications for tissue regeneration and healing.
10:25
Microfluidic Flow Chambers Using Reconstituted Blood to Model Hemostasis and Platelet Transfusion In Vitro
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Cited by 9 •
2016
Platelet transfusion and hemostasis was modeled using blood reconstitution and microfluidic flow chambers to investigate the function of blood banking platelets. The data demonstrate the consequences of platelet storage lesion on hemostasis, in vitro.
05:41
Purification of Platelets from Mouse Blood
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Cited by 9 •
2019
Here, mouse blood was collected in the presence of an anti-coagulant. The platelets were purified by iohexol gradient medium using low speed centrifugation. The platelets were activated with thrombin to investigate if they were viable. The quality of the purified platelets was analyzed by flow cytometry and microscopy.
09:38
A Microfluidic Flow Chamber Model for Platelet Transfusion and Hemostasis Measures Platelet Deposition and Fibrin Formation in Real-time
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Cited by 13 •
2017
This paper describes an experimental model of hemostasis that simultaneously measures platelet function and coagulation. Platelet and fibrin fluorescence is measured in real-time, and platelet adhesion rate, coagulation rate, and onset of coagulation are determined. The model is used to determine platelet procoagulant properties under flow in concentrates for transfusion.
FAQs
At vessel injury, exposed collagen provides the surface for platelet adhesion. Von Willebrand factor enables this interaction, helping platelets attach where vascular integrity has been challenged. This localization is important because adhesion is the entry point for the subsequent activation sequence, rather than an isolated event in circulation.
Once platelets attach, they activate, change shape, and release granule contents. These events create a recruitment signal that brings additional platelets to the injury and also supports coagulation. The sequence explains how a local vessel-wall response expands into a platelet plug, while preserving the distinction between initial adhesion and later activation-driven amplification.
Platelet recruitment and granule release help build the plug at an injured vessel, while fibrin stabilizes that plug. This relationship links cellular activity with coagulation: platelet responses form the plug, while fibrin stabilizes the completed structure. Examining both elements gives a more complete view of how vascular damage is contained than studying platelet activity alone.
Platelet count and function provide different kinds of biological information. Count indicates the platelet measure available in circulation, while function concerns their participation in adhesion, activation, recruitment, and coagulation. Considering these indicators in relation to hemostasis, inflammation, and disease helps researchers and clinicians interpret platelet-related changes without treating number as the only relevant measure.
Platelet research addresses both inadequate and excessive clotting outcomes. In bleeding disorders, investigators examine how platelet-related processes may fail to limit blood loss; in thrombosis and cardiovascular disease, they study platelet and clot-forming activity in relation to disease processes. These contrasting applications make platelets relevant to disease mechanisms as well as medical research.
In transfusion practices, platelet biology matters because platelet count and function are relevant to hemostasis. Research also uses platelets when developing or evaluating therapies that regulate clot formation. The same biological system can therefore be considered from two practical directions: supporting control of blood loss and limiting inappropriate clotting, depending on the medical context.