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Purification of Platelets from Mouse Blood
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Purification of Platelets from Mouse Blood

Purification of Platelets from Mouse Blood

DOI:
10.3791/59803-v

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05:41 min

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May 07, 2019

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,
1Division of Experimental Hematology and Cancer Biology,Cincinnati Children’s Hospital Medical Center, 2University of Cincinnati College of Medicine

Chapters

  • 00:04Title
  • 00:37Platelet Purification
  • 01:31Platelet Activation
  • 02:51Flow Cytometric Platelet Analysis
  • 03:30Results: Representative Platelet Purification Evaluation
  • 05:03Conclusion

Summary

Automatic Translation

Automatic Translation

Here, mouse blood was collected in the presence of an anti-coagulant. The platelets were purified by iohexol gradient medium using low speed centrifugation. The platelets were activated with thrombin to investigate if they were viable. The quality of the purified platelets was analyzed by flow cytometry and microscopy.

Tags

Keywords: Platelet PurificationMouse BloodIohexol GradientLow-speed CentrifugationPlatelet-rich LayerPlatelet ActivationGPRP PeptideThrombinFlow CytometryForward ScatterSide Scatter

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