Cancer Diseases
Cancer Diseases - Related Videos
07:16
Isolation of Cancer Stem Cells From Human Prostate Cancer Samples
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Cited by 6 •
2014
The isolation of cancer stem cells (CSCs) directly from human tissues is requisite for their biological characterization. This manuscript describes a methodology for the isolation of prostate CSCs from human tissues, while also providing tips on troubleshooting difficult steps.
10:47
An Overview of Genetics and Disease
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2023
Many human diseases are associated with mutations or variations in genetic sequences. Some of these genetic variants are heritable, passed down from generation to generation, while others arise sporadically during an organism’s life and cause diseases such as cancer. Researchers are trying to identify and characterize these genetic alterations in the hopes of improving diagnosis and therapeutic options for patients.In this video, we will examine the history of genetic disease research, and...
09:36
Human Neural Organoids for Studying Brain Cancer and Neurodegenerative Diseases
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Cited by 14 •
2019
This study introduces and describes protocols to derive two specific human neural organoids as a relevant and accurate model for studying 1) human glioblastoma development within human neural organoids exclusively in humans and 2) neuron dopaminergic differentiation generating a three-dimensional organoid.
13:18
How to Study Basement Membrane Stiffness as a Biophysical Trigger in Prostate Cancer and Other Age-related Pathologies or Metabolic Diseases
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Cited by 4 •
2016
Here we explain a protocol for modelling the biophysical microenvironment where crosslinking and increased stiffness of the basement membrane (BM) induced by advanced glycation endproducts (AGEs) has pathological relevance.
10:58
Quantitation of Intra-peritoneal Ovarian Cancer Metastasis
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Cited by 15 •
2016
Ovarian cancer metastasis is characterized by numerous diffuse intra-peritoneal lesions, such that accurate visual quantitation of tumor burden is challenging. Herein we describe a method for in situ and ex vivo quantitation of metastatic tumor burden using red fluorescent protein (RFP)-labeled tumor cells and optical imaging.
FAQs
Genetic and epigenetic changes can disrupt the controls governing cell division, DNA repair, apoptosis, and communication with neighboring cells. Because these controls coordinate whether cells divide, repair damage, persist, or respond to local signals, their disruption provides a biological basis for abnormal cell expansion. Cancer biology therefore examines both forms of change when investigating tumor development.
Apoptosis is a programmed route for cell removal. When this process is disrupted, abnormal cells may avoid a normal means of elimination. Impaired DNA repair can also leave damaging changes uncorrected, while altered signaling with neighboring cells can disturb tissue-level control. Examining these mechanisms clarifies how multiple cellular failures can cooperate during tumor development.
Local invasion concerns movement into nearby tissues, whereas metastasis refers to spread to other parts of the body. Studying them separately helps biology examine how tumor cells interact with surrounding tissue and how disease extends beyond its original site. This distinction is important when evaluating tumor progression and considering how treatment responses may differ across disease stages.
The immune system is studied as part of the tumor environment and as a factor in treatment response. Cancer biology examines interactions between tumors and immune defenses to understand how those relationships influence tumor development and immunotherapy. This perspective broadens analysis beyond abnormal cells themselves and supports approaches intended to target disease while limiting harm to healthy tissue.
Targeted therapies and immunotherapies represent different treatment-oriented approaches. Targeted therapies are designed to inhibit tumor growth, whereas immunotherapies are studied in relation to the immune system and tumor interactions. Comparing these approaches helps investigators consider how treatment can act against cancer while limiting damage to healthy tissue. Their effectiveness is also examined through responses to treatment.
Cancer research connects biological changes and tumor behavior with practical goals such as earlier diagnosis, prevention, and treatment development. Investigations of tumor development, immune interactions, angiogenesis, metastasis, and treatment responses can reveal which aspects of disease should be detected or controlled. These findings support therapies and other strategies intended to inhibit tumor growth while limiting damage to healthy tissue.