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How to Study Basement Membrane Stiffness as a Biophysical Trigger in Prostate Cancer and Other Age-related Pathologies or Metabolic Diseases
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
How to Study Basement Membrane Stiffness as a Biophysical Trigger in Prostate Cancer and Other Age-related Pathologies or Metabolic Diseases

How to Study Basement Membrane Stiffness as a Biophysical Trigger in Prostate Cancer and Other Age-related Pathologies or Metabolic Diseases

DOI:
10.3791/54230-v

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13:18 min

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September 20, 2016

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, , , , , , , , ,
1Departamento de Genética, Facultad de Medicina,Universidad de la República (UDELAR), 2Department of Mechanistic Cell Biology,Max Planck Institute of Molecular Physiology, 3School of Biological, Biomedical & Environmental Sciences,University of Hull

Chapters

  • 00:05Title
  • 01:38Induction of Basement Membrane Stiffness Induced by GLA Treatment
  • 04:18Photometric and SDS-PAGE Analysis of Reconstituted Basement Membrane
  • 06:27Immunofluorescent Microscopy Analysis
  • 09:093D Culture of Prostate Gland Acini and Prostate Tumor Cell Aggregates on Native and Stiff Reconstituted Basement Membrane
  • 10:41Results: Prostate Cell Reactions to Changes in Basement Membrane Stiffness
  • 12:02Conclusion

Summary

Automatic Translation

Automatic Translation

Here we explain a protocol for modelling the biophysical microenvironment where crosslinking and increased stiffness of the basement membrane (BM) induced by advanced glycation endproducts (AGEs) has pathological relevance.

Tags

Keywords: Basement MembraneStiffnessThicknessAdvanced GlycationProstate CancerMetastasisAge-related PathologiesMetabolic DiseasesCell Invasion3D Cell CultureCross-linkingBiophysical Trigger

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