Carbon-14 Palmitic Acid
Carbon-14 Palmitic Acid - Related Videos
01:22
Bicarbonate-Carbonic Acid Buffer
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2024
The carbonic acid-bicarbonate buffer system is critical for maintaining the body's pH balance. It operates on the equilibrium: H2CO3 ⇋ H+ + HCO3- In this system, bicarbonate ions (HCO3⁻) act as weak bases, and carbonic acid (H₂CO₃) serves as a weak acid. This dynamic equilibrium enables the system to respond effectively to changes in pH. When hydrogen ion (H+) levels increase, causing a drop in pH, the equilibrium shifts to the left, converting hydrogen ions into carbonic acid. The enzyme...
10:01
Measurement of Carbon Dioxide Production from Radiolabeled Substrates in Drosophila melanogaster
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Cited by 7 •
2016
This paper describes a method for the measurement of fuel oxidation in Drosophila melanogaster in which trace amounts of specific radiolabeled metabolic substrates are fed to flies. The exhaled radiolabeled CO2 that is a produced from fuel oxidation is collected and measured.
08:12
Characterization, Quantification and Compound-specific Isotopic Analysis of Pyrogenic Carbon Using Benzene Polycarboxylic Acids (BPCA)
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Cited by 40 •
2016
We present the benzene polycarboxylic acid (BPCA) method for assessing pyrogenic carbon (PyC) in the environment. The compound-specific approach uniquely provides simultaneous information about the characteristics, quantity and isotopic composition (13C and 14C) of PyC.
12:11
Determination of Fatty Acid Oxidation and Lipogenesis in Mouse Primary Hepatocytes
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Cited by 39 •
2015
De novo lipogenesis and β-fatty acid oxidation constitute key metabolic pathways in hepatocyte, pathways that are perturbed in several metabolic disorders, including fatty liver disease. Here we demonstrate isolation of mouse primary hepatocytes and describe quantification of β-fatty acid oxidation and lipogenesis.
01:14
The Carbon Cycle
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2019
Carbon is the basis of all organic matter on Earth, and is recycled through the ecosystem in two primary processes: one in which carbon is exchanged among living organisms, and one in which carbon is cycled over long periods of time through fossilized organic remains, weathering of rocks, and volcanic activity. Human activities, including increased agricultural practices and the burning of fossil fuels, has greatly affected the balance of the natural carbon cycle. Biological Carbon Cycle All...
FAQs
After uptake, cells may incorporate the labeled fatty acid into lipid stores or activate it to palmitoyl-CoA. The activated form can enter mitochondrial beta-oxidation, a pathway that removes two-carbon acetyl-CoA units successively. Tracking the radioactive carbon through this process helps connect fatty acid handling with downstream oxidation and energy production.
The signal can provide separate information about fatty acid uptake, incorporation, and oxidation, rather than indicating only that the molecule entered a cell. Comparing these metabolic outcomes shows whether biological systems are storing the fatty acid, processing it through beta-oxidation, or using it in pathways associated with energy production.
Storage and oxidation represent different metabolic fates for the same fatty acid. Incorporation into lipid stores indicates retention within cellular lipid pools, whereas conversion to palmitoyl-CoA followed by beta-oxidation indicates utilization through successive acetyl-CoA release. Distinguishing these fates helps researchers determine how cells balance lipid accumulation against lipid use.
Nutrition, hormones, disease, and experimental treatments can alter how biological systems handle the labeled fatty acid. Changes may appear in its uptake, incorporation into lipid stores, oxidation, or relationship to energy production. Measuring these outcomes under different conditions allows researchers to identify how broader physiological or pathological states reshape lipid utilization.
Researchers apply the tracer in cultured cells, tissues, or organisms and examine the resulting radioactive signal. The measurements can be organized around uptake, incorporation, oxidation, and energy production, depending on the study’s objective. This approach provides a way to compare lipid handling across biological systems or experimental conditions without treating all fatty acid processing as one outcome.
This tracer supports investigations of how lipid metabolism responds to nutrition, hormonal influences, disease, or experimental treatments. By following labeled carbon through storage and oxidation-related outcomes, researchers can clarify metabolic pathways and determine whether a condition changes fatty acid acquisition, retention, or utilization. Such comparisons connect biochemical processing with broader biological changes.