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Investigating the Protective Effects of Platycodin D on Non-Alcoholic Fatty Liver Disease in a Palmitic Acid-Induced In Vitro Model
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Investigating the Protective Effects of Platycodin D on Non-Alcoholic Fatty Liver Disease in a Palmitic Acid-Induced In Vitro Model

Investigating the Protective Effects of Platycodin D on Non-Alcoholic Fatty Liver Disease in a Palmitic Acid-Induced In Vitro Model

DOI:
10.3791/64816-v

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08:20 min

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December 02, 2022

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, , , , , , ,
1Chongqing Academy of Chinese Materia Medica, 2Bioengineering College of Chongqing University

Chapters

  • 00:05Introduction
  • 00:58Pretreatment of AML-12 Cells for In Vitro NAFLD Model
  • 02:32Measuring the Change in ROS Production and Mitochondrial Membrane Potential
  • 04:05Measuring the Protein Expression Levels of LC3-II/LC3-I and p62/SQSTM1
  • 05:59Results: Effects of Platycodin D on Intracellular ROS, MMP Depolarization, and Protein Expression Levels in AML-12 Cells
  • 07:49Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol investigates the protective effects of platycodin D on non-alcoholic fatty liver disease in a palmitic acid-induced in vitro model.

Tags

Keywords: AML-12 CellsNon-alcoholic Fatty Liver Disease (NAFLD)Platycodin DPalmitic AcidCell ModelIn VitroOxidative StressMitochondrial Membrane PotentialMicroscopy

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