Cell Surface Imaging
Cell Surface Imaging - Related Videos
11:59
High-speed Particle Image Velocimetry Near Surfaces
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Cited by 19 •
2013
A procedure for studying transient flows near boundaries using high-resolution, high-speed particle image velocimetry (PIV) is described here. PIV is a non-intrusive measurement technique applicable to any optically accessible flow by optimizing several parameter constraints such as the image and recording properties, the laser sheet properties, and analysis algorithms.
05:00
Localized Surface Plasmon Resonance Imaging to Detect Protein Secretions from a Single Cell
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2025
This video demonstrates localized surface plasmon resonance imaging to detect protein secretions from a single cell. Using a microfluidic setup and a peptide-bound plasmonic nanostructure chip, changes in reflected light intensity at the resonance angle reveal interactions with secreted proteins from a single cell.
08:49
Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens
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Cited by 8 •
2020
This manuscript describes a genome-scale cell-based screening approach to identify extracellular receptor-ligand interactions.
01:21
Cell-surface Signaling
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2019
Hormones—or any molecule that binds to a receptor, known as a ligand—that are lipid-insoluble (water-soluble) are not able to diffuse across the cell membrane. In order to be able to affect a cell without entering it, these hormones bind to receptors on the cell membrane. When a first messenger, a hormone, binds to a receptor, a signal cascade is set off, causing second messengers, proteins inside the cell, to become activated, resulting in downstream effects. Variety of Receptor Families
06:31
Real-time Imaging of Plant Cell Surface Dynamics with Variable-angle Epifluorescence Microscopy
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Cited by 10 •
2015
The goal of this protocol is to demonstrate how to monitor fluorescently-tagged protein dynamics on plant cell surfaces with variable-angle epifluorescence microscopy, showing blinking dots of GFP-tagged PATROL1, a membrane trafficking protein, in the cell cortex of the stomatal complex in Arabidopsis thaliana.
FAQs
These labels bind to, or localize near, selected membrane components, making those components detectable through light, fluorescence, or high-resolution microscopy. Their signals can show where receptors and other surface molecules are positioned on individual cells. This spatial information helps connect molecular organization with cellular identity, communication, and interactions with the surrounding environment.
Fluorescent antibodies, molecular probes, and tagged proteins provide different ways to mark membrane components. The selected approach determines which surface feature becomes visible and how its location can be examined. Comparing signals from different labels can therefore help researchers distinguish receptors, other membrane molecules, and specific patterns associated with cellular behavior or identity.
Patterns at the membrane can show how receptors and other surface molecules are arranged where cells contact one another or interact with their surroundings. These observations provide a visual connection between surface organization and processes such as signaling and immune recognition. They can also help researchers examine how molecular positioning relates to cell-cell interactions.
Differences in surface molecules and their organization can produce distinct imaging patterns among healthy, developmental, or diseased cells. By mapping these features, researchers can compare cellular states rather than relying only on overall appearance. The resulting information supports studies of disease-associated changes, cellular development, and the identification of surface features relevant to therapeutic targeting.
A typical workflow begins by selecting a fluorescent antibody, molecular probe, or tagged protein that binds to or localizes with the membrane component of interest. Researchers then detect the label using light, fluorescence, or high-resolution microscopy. The resulting images can be analyzed to map receptors, examine surface organization, and relate membrane features to cell behavior.
Depending on the label and microscopy approach, the method can provide maps of receptors and other surface molecules, observations of membrane dynamics, or visual records of cell-cell interactions. These outcomes support investigations of signaling, immune recognition, infection, and therapeutic targeting. In each case, the images help relate surface-level molecular patterns to broader biological activity.