Blastocyst Formation
Blastocyst Formation - Related Videos
10:59
Human Blastocyst Biopsy and Vitrification
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Cited by 36 •
2019
Blastocyst biopsy and vitrification are required to efficiently conduct preimplantation genetic testing. An approach entailing the sequential opening of the zona pellucida and retrieval of 7-8 trophectoderm cells in day 5-7 post-insemination limits both the number of manipulations required and the exposure of the embryo to sub-optimal environmental conditions.
10:19
Derivation of Mouse Trophoblast Stem Cells from Blastocysts
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Cited by 7 •
2010
In this video, we demonstrate the isolation of mouse blastocysts and the derivation of trophoblast stem cells from blastocysts. We also describe conditions for maintenance of the stem cell property as well as induction of differentiation in culture.
12:09
Protocol for Human Blastoids Modeling Blastocyst Development and Implantation
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Cited by 6 •
2022
A protocol outlining the formation of human blastoids that efficiently, timely, and sequentially generate blastocyst-like cells.
02:16
Solution Formation
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2020
There is no one solvent that can dissolve every type of solute. Some substances that readily dissolve in a certain solvent might be insoluble in a different solvent. A simple way to predict which substances dissolve in which solvent is the phrase "like dissolves like". This means that polar substances, such as salt and sugar, dissolve in a polar substance like water. In contrast, non-polar substances are more soluble in non-polar solvents such as carbon tetrachloride. This selective solubility...
09:35
Modified MicroSecure Vitrification: A Safe, Simple and Highly Effective Cryopreservation Procedure for Human Blastocysts
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Cited by 11 •
2017
MicroSecure Vitrification was developed as a non-commercial, aseptic closed vitrification device system that is compliant with FDA good manufacturing and tissue-handling practices. Due to the withdrawal of hydrophobic plugged embryo straws from the industry, the vitrification procedure was modified to include an inner seal before the standard internal cotton plug.
FAQs
Repeated cleavage divisions increase the number of embryonic cells, while compaction brings those cells into a more organized arrangement. This reorganization is important because it precedes the separation of two developmental populations: the outer trophectoderm and the inner cell mass. Together, these changes establish the cellular organization required for later blastocyst development.
Fluid accumulation produces the blastocoel, the internal cavity that gives the developing structure its fluid-filled organization. Its appearance marks a major architectural change after cell compaction and lineage separation. This cavity is therefore useful for recognizing progression toward the blastocyst stage and for studying how early embryonic structure becomes prepared for implantation.
The two cell populations have distinct developmental roles. The trophectoderm forms the outer layer, supports interaction with the uterine lining, and contributes to extraembryonic tissues. The inner cell mass remains associated with formation of the embryo proper. Examining their separation helps researchers connect early cell organization with later tissue and embryo development.
Researchers can follow a sequence of observable changes: repeated cleavage, compaction, separation into an outer trophectoderm and inner cell mass, and accumulation of fluid to produce the blastocoel. Assessing these features links cell division, organization, differentiation, and cavity formation, providing a framework for investigating regulation during early mammalian development.
This developmental transition provides a model for examining how early embryonic cells become differentiated and organized. Studies can focus on the relationship between cellular changes and developmental regulation, while also connecting these processes to the embryo's preparation for implantation. The same research context informs investigations of infertility and early mammalian development.
Blastocyst formation is relevant because it links early embryonic development with preparation for implantation in the uterus. Investigating cleavage, compaction, lineage separation, and blastocoel formation can help researchers understand developmental progression in contexts related to infertility and assisted reproductive technologies. These observations provide a biological basis for evaluating early embryonic development.