Cell Transplantation
Cell Transplantation - Related Videos
09:24
Molecular Imaging to Target Transplanted Muscle Progenitor Cells
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Cited by 3 •
2013
A non-invasive means to evaluate the success of myoblast transplantation is described. The method takes advantage of a unified fusion reporter gene composed of genes whose expression can be imaged with different imaging modalities. Here, we make use of a fluc reporter gene sequence to target cells via bioluminescence imaging.
08:15
Cervical Heterotopic Mouse Heart Transplantation using End-to-side Anastomosis Technique to Study Transplant Immunology
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2026
This protocol presents a simplified, end-to-side suture anastomosis technique for murine cervical heart transplantation, providing a robust and reproducible model for studying transplant immunology.
06:04
Transplantation of Cells Directly into the Kidney of Adult Zebrafish
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Cited by 10 •
2011
Cell transplantation is an essential technique for studying tissue regeneration and for developing cell-based therapies of disease. We demonstrate here a microsurgical technique that permits the transplantation of genetically labeled cells directly into the kidney of adult zebrafish fish.
08:01
Quantifying the Frequency of Tumor-propagating Cells Using Limiting Dilution Cell Transplantation in Syngeneic Zebrafish
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Cited by 35 •
2011
Limiting dilution cell transplantation assays are used to determine the frequency of tumor-propagating cells. This protocol describes a method for generating syngeneic zebrafish that develop fluorescently-labeled leukemia and details how to isolate and transplant these leukemia cells at limiting dilution into the peritoneal cavity of adult zebrafish.
04:05
Subcutaneous Transplantation Assay: A Method to Transplant Melanoma Cells from a Donor to a Recipient Zebrafish Model
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2023
This video describes the method for transplantation of tumor cells from the melanoma-bearing donor zebrafish model to an immunocompromised recipient Casper zebrafish. It helps to visualize tumor engraftment and proliferation in the recipient fish.
FAQs
Transplanted cells may support recovery through several complementary outcomes. Some survive and engraft within the recipient tissue, while others communicate with nearby cells or secrete signaling molecules that influence repair. In certain settings, the cells may also differentiate, meaning they acquire specialized characteristics. These different responses help explain why transplantation can affect biological function even when complete replacement does not occur.
Survival allows transferred cells to remain viable after delivery, while engraftment indicates that they become established within the recipient tissue. Both outcomes affect whether cells can maintain communication, release signaling molecules, or contribute to differentiated tissue functions. Poor survival or limited engraftment can reduce the biological effect, making these outcomes central to evaluating transplantation strategies.
Compatibility and host immune responses can determine whether transplanted cells persist and function effectively. The recipient environment may recognize transferred cells as biologically different, influencing their survival and integration. Consequently, researchers consider the cell source and the recipient context when interpreting outcomes. These factors are especially relevant when comparing transplantation results across organisms, tissues, or experimental conditions.
Cell delivery may occur through injection, infusion, or implantation, with the choice linked to the intended recipient tissue or biological objective. Researchers also consider the delivery site because local conditions can affect survival, engraftment, communication with surrounding cells, and functional recovery. Evaluating the route together with the target tissue helps connect the procedure to its expected biological outcome.
Cell transplantation supports both therapeutic and investigative research. Hematopoietic stem cell transplantation represents an important application, while experimental studies examine possible approaches to degenerative disease and tissue regeneration. In biology, transplanted cells also provide models for studying immune responses, development, cellular behavior, and interactions between introduced cells and the surrounding tissue environment.
Evaluation centers on whether transplanted cells survive, engraft, communicate with the surrounding microenvironment, differentiate, or promote functional recovery. Researchers interpret these outcomes alongside the cell source, delivery site, vascularization, compatibility, and host immune responses. This multifactorial assessment helps distinguish simple cell persistence from broader biological effects and guides efforts to improve integration and tissue repair.