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Immunology and Infection
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Normal and Malignant Muscle Cell Transplantation into Immune Compromised Adult Zebrafish
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Normal and Malignant Muscle Cell Transplantation into Immune Compromised Adult Zebrafish

Normal and Malignant Muscle Cell Transplantation into Immune Compromised Adult Zebrafish

DOI:
10.3791/52597-v

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09:39 min

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December 26, 2014

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, , ,
1Molecular Pathology, Cancer Center and Center for Regenerative Medicine,Massachusetts General Hospital, 2Harvard Stem Cell Institute, 3GABBA – Instituto de Ciências Biomédicas Abel Salazar,Universidade do Porto

Chapters

  • 00:05Title
  • 02:01Preparation of Adult Zebrafish Donor Skeletal Muscle Cells
  • 03:57Intramuscular Transplantation of Skeletal Muscle Cells into Adult Zebrafish
  • 05:02Embryonal Rhabdomyosarcoma (ERMS) Tumor Preparation and Transplantation
  • 07:04Results: Skeletal Muscle Engraftment
  • 08:39Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a protocol for cell transplantation of zebrafish skeletal muscle and embryonal rhabdomyosarcoma (ERMS) into adult immune compromised rag2E450fs homozygous mutant zebrafish. This protocol allows for the efficient analysis of regeneration and malignant transformation of muscle cells.

Tags

Keywords: ZebrafishCell TransplantationSkeletal MuscleRhabdomyosarcomaRag2 MutantImmune CompromisedEngraftmentRegenerationCancer

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