Cheek Cell Collection
Cheek Cell Collection - Related Videos
04:59
Cheek Injection Model for Simultaneous Measurement of Pain and Itch-related Behaviors
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Cited by 13 •
2019
Typically, the mouse neck injection model is used for evaluate pruritogen-induced scratch behaviors. However, the model provides information only on itch, not pain. Here, a cheek injection model is introduced in mice which can be used to simultaneously measure pain and itch-related behaviors.
06:11
Culturing Primary Rat Inner Medullary Collecting Duct Cells
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Cited by 20 •
2013
Arginine-vasopressin (AVP) controls fine-tuning of body water homeostasis through facilitating water reabsorption by renal principal cells. Here, we present a protocol for the cultivation of primary rat inner medullary collecting duct cells suitable for the elucidation of molecular mechanisms underlying AVP-mediated water reabsorption.
02:45
Production and Collection of Pseudovirus Particles Using Mammalian Cell Culture
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2026
Source: Jamieson, T. R., et al. Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection. J. Vis. Exp. (2021)This video demonstrates the procedure for infecting mammalian cells with green fluorescent protein (GFP)-encoding pseudovirus, monitoring viral replication and spread by fluorescence microscopy, and collecting virus-containing supernatant for downstream applications.
07:08
Modeling and Imaging 3-Dimensional Collective Cell Invasion
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Cited by 20 •
2011
Models of tumor cell invasion into three-dimensional extracellular matrix better reflect the in vivo situation than two-dimensional motility assays. Using matrix invasion assays combined with confocal imaging of fluorescently-labeled cells, detailed information on invasion modes and the distinct contributions of leading versus following cells can be obtained.
08:23
Seawater Sampling and Collection
0 Views •
Cited by 13 •
2009
This video documents methods for collecting coastal marine water samples and processing them for various downstream applications including biomass concentration, nucleic acid purification, cell abundance, nutrient and trace gas analyses.
FAQs
Gentle rubbing of the inner cheek loosens buccal epithelial cells from the mouth’s surface. The collection device captures these cells and transfers them to a slide or tube, depending on the planned analysis. Because the sample contains intact human cells, it can support either examination of cellular features or later processing to isolate DNA.
The inner cheek provides a directly accessible surface containing buccal epithelial cells, allowing sampling without an invasive procedure. Rubbing this surface helps release cells for capture, while the use of a sterile device helps limit unwanted material. Consistent contact with the cheek supports a sample suitable for the intended downstream laboratory analysis.
The collected material can follow different processing paths. For DNA-based work, cells may be processed to isolate their DNA for applications such as DNA profiling or genetics education. For cellular analysis, the sample may be transferred to a slide so researchers can examine cellular features. The container or surface should match the planned laboratory method.
Reliable results depend on obtaining an adequate sample and preserving its identity during handling. Gentle but effective rubbing helps dislodge cells, while sterile collection equipment reduces contamination risk. Accurate labeling connects each sample with the correct source, and careful transfer to a tube or slide supports the analysis selected for the experiment.
A basic workflow uses a sterile swab or comparable collection device to gently rub the inner cheek, then transfers the collected material to a labeled tube or slide. The sample is subsequently directed toward DNA isolation or examination of cellular features. Maintaining clean handling throughout the collection and transfer stages helps protect the usefulness of the specimen.
Sterile equipment helps reduce contamination that could interfere with laboratory analysis, while accurate labeling prevents samples from being confused during processing. These safeguards are especially important when multiple specimens are handled or when results will be compared. Together, they support reliable interpretation of DNA-based findings or observations of cellular features.
This approach is useful when investigators or students need human cellular material without blood collection. Supported applications include genetics education, DNA profiling, disease-related research, and studies of human variation. Its accessible sampling approach can simplify specimen acquisition while still providing material for DNA processing or examination of cheek-cell features.