Chick Embryo Culture
Chick Embryo Culture - Related Videos
08:45
Method for Culture of Early Chick Embryos ex vivo (New Culture)
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Cited by 9 •
2008
This video demonstrates New culture, a method by which chick embryos are cultured outside the egg for up to 24 hr. This method enables one to study early development (primitive streak to 14 som.), a period corresponding to E7-9 in mouse. Applications of this technique include electroporation, in situ hybridization and immunohistochemistry.
06:48
Chick ex ovo Culture
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2023
One strength of the chicken (Gallus gallus domesticus) as a model organism for developmental biology is that the embryo develops outside the female and is easily accessible for experimental manipulation. Many techniques allow scientists to examine chicken embryos inside the eggshell (in ovo), but embryonic access can be limited at later stages of development. Fortunately, chicks can also be cultured ex ovo, or outside of the eggshell. The major advantage to ex ovo culture is greater access to...
08:19
Tracking Morphogenetic Tissue Deformations in the Early Chick Embryo
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Cited by 11 •
2011
This article describes surface labeling and ex ovo tissue culture in the early chick embryo. Techniques amenable to time-lapse bright field, fluorescence, and optical coherence tomography imaging are presented. Tracking surface labels with high spatiotemporal resolution enables kinematic quantities such as morphogenetic strains (deformations) to be calculated in both two and three dimensions.
03:34
Isolating Cranial Neural Folds From a Chick Embryo for a Neural Crest Cell Culture
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2025
This video illustrates a technique for isolating cranial neural folds from the midbrain region of chick embryos. It presents a detailed dissection process of the neural folds, their placement, and attachment on fibronectin-coated coverslips, followed by the observation of neural crest cell migration from the neural folds.
11:14
Assay for Neural Induction in the Chick Embryo
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Cited by 1 •
2009
Neural induction is the first step in the formation of the brain. It is a mechanism by which Hensen's node (organizer), instructs adjacent tissue to adopt a neural fate, i.e. to give rise to the nervous system. This video demonstrates an assay for neural induction in chick embryo.
FAQs
Successful culture depends on maintaining several interacting requirements rather than temperature alone. The sterile system must provide access to nutrients, support gas exchange, limit excessive water loss, and maintain suitable hydration and temperature. Preserving these conditions allows the embryo and its supporting membranes to continue developmental processes that would otherwise occur within the intact egg.
The supporting membranes remain important because they help connect the developing embryo with the conditions required for growth. Their presence supports access to nutrients, gas exchange, and hydration within the culture system. Keeping these structures associated with the embryo helps researchers observe development while retaining biological support for processes such as tissue formation and blood-vessel development.
Direct observation reveals morphogenesis, the shaping of developing tissues, as it occurs rather than only showing a final outcome. Researchers can follow cell migration, tissue interactions, and changes in organ formation over time. This visibility makes the model useful for linking developmental events with experimental manipulations or altered environmental and molecular signals.
Preparation requires a sterile culture system with controlled temperature, hydration, nutrient access, and gas exchange. These conditions work together to support continued growth and reduce disruptions caused by contamination or unsuitable surroundings. Attention to this combination is essential because the culture must provide developmental support while also keeping the embryo accessible for observation and experimentation.
The general workflow begins by transferring the embryo and its supporting membranes from the intact egg into a sterile culture system. The system is then maintained under conditions that provide nutrients, gas exchange, hydration, and temperature control. During culture, researchers observe development and may examine responses to experimental manipulation, including changes in tissues, migration, or organ formation.
Cultured chick embryos support questions about how vertebrate tissues form, move, and interact during development. They are particularly useful for examining morphogenesis, cell migration, tissue interactions, organ formation, and blood-vessel development. Researchers can also evaluate how environmental or molecular signals influence these processes, making the model relevant to broad developmental biology studies.